Instrument-Free Protein Microarray Fabrication for Accurate Affinity Measurements

Protein microarrays have gained reputation as a lovely instrument for numerous fields, together with drug and biomarker improvement, and diagnostics. Thus, multiplexed binding affinity measurements in microarray format has change into essential. The preparation of microarray-based protein assays depends on exact shelling out of probe options to attain environment friendly immobilization onto an lively floor.

The prohibitively excessive price of kit and the necessity for educated personnel to function excessive complexity robotic spotters for microarray fabrication are vital detriments for researchers, particularly for small laboratories with restricted sources. Right here, we current a low-cost, instrument-free shelling out method by which customers who’re conversant in micropipetting can manually create multiplexed protein assays that present improved seize effectivity and noise stage compared to that of the robotically noticed assays.

On this examine, we examine the effectivity of manually and robotically distributed α-lactalbumin probe spots by analyzing the binding kinetics obtained from the interplay with anti-α-lactalbumin antibodies, utilizing the interferometric reflectance imaging sensor platform. We present that the protein arrays ready by micropipette guide recognizing meet and exceed the efficiency of these ready by state-of-the-art robotic spotters.

These instrument-free protein assays have the next binding sign (~4-fold enchancment) and a ~3-fold higher signal-to-noise ratio (SNR) in binding curves, when in comparison with the information acquired by averaging 75 robotic spots comparable to the identical efficient sensor floor space. We exhibit the potential of figuring out antigen-antibody binding coefficients in a 24-multiplexed chip format with lower than 5% measurement error. Fractionation data on arsenic (As) in complicated samples, notably stable samples, is of immense curiosity. Herein, selective extraction of varied As species adsorbed onto ferrihydrite because the mannequin substrate was online-adapted to inductively coupled plasma-mass spectrometry (ICP-MS) for delicate detection.

The As-adsorbed ferrihydrite pattern was loaded right into a home made on-line sequential elution machine utilizing two commercially obtainable micropipette ideas, after which, every fraction of As together with nonspecifically adsorbed, particularly adsorbed, iron oxide bonded, and residual species was successively extracted for ICP-MS detection, with H2O, NH4NO3, NH4H2PO4, ammonium oxalate, and HF because the eluents, respectively.

Constitutive Mannequin of Erythrocyte Membranes with Distributions of Spectrin Orientations and Lengths

We current an analytical hyperelastic constitutive mannequin of the pink blood cell (erythrocyte) membrane based mostly on lately improved characterizations of density and microscopic construction of its spectrin community from proteomics and cryo-electron tomography. The mannequin consists of distributions of each orientations and pure lengths of spectrin and up to date copy numbers of proteins.

By making use of finite deformation to the spectrin community, we receive the entire free vitality and stresses by way of invariants of shear and space deformation. We generalize an expression of the preliminary shear modulus, which is impartial of the variety of molecular orientations inside the community and likewise derive a simplified model of the mannequin.

We apply the mannequin and its simplified model to investigate micropipette aspiration computationally and analytically, and discover the impact of native cytoskeletal density change. We additionally discover the discrepancies amongst shear modulus values measured utilizing completely different experimental strategies reported within the literature.

We discover that the mannequin displays hardening habits and might clarify many of those discrepancies. Furthermore, we discover that the distribution of pure lengths performs an important position within the hardening habits when the proper copy numbers of proteins are used. The preliminary shear modulus values we receive utilizing our present mannequin (5.9∼15.6 pN/μm) are near the early estimates (6-9 pN/μm). This new constitutive mannequin establishes a direct connection between the molecular construction of spectrin networks and constitutive legal guidelines, and likewise defines a brand new image of a a lot denser spectrin community than assumed in prior research.

 Instrument-Free Protein Microarray Fabrication for Accurate Affinity Measurements
Instrument-Free Protein Microarray Fabrication for Accurate Affinity Measurements

Newly designed dual-mode electrochemical sensor onto a single polydimethylsiloxane-based chip

Right here we current a planar polydimethylsiloxane (PDMS)-based millifluidic machine during which the channel (at a serpentine-shape) and a brand new design of electrochemical sensor (dual-mode detection) are assembled right into a single chip. The platform makes use of a totally built-in reservoir to put the reference electrode, separating it from the flowing stream.

The machine was correctly characterised aiming to judge the influences of channel geometries and diameters, and the electrochemical properties have been improved in contrast with the classical reference electrode configuration. To analyze the applicability of the proposed millifluidic machine, amperometric detection was used for the evaluation of faucet and lake water in addition to groundwater samples to find out salicylic acid – chosen as a mannequin analyte.

Magnetic Stand

HY-K0200 200 μL-2 mL-15 mL
EUR 380

Magnetic Stand

abx799001-1Unit 1 Unit
EUR 258

CAROUSEL STAND FOR AXYPET

AP-CSD 1/pk
EUR 133
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

stand for 15 w lamps

VLS30 ea
EUR 200

SureStand Pipette Stand for 3 pipettes, up to one multi-channel, acrylic

P4403 1/pack
EUR 81.05
Description: SureStand Pipette Stand for 3 pipettes up to one multi-channel

SureStand Pipette Stand for 5 pipettes, up to two multi-channels, acrylic

P4405 1/pack
EUR 89.15
Description: SureStand Pipette Stand for 5 pipettes up to two multi-channels

SureStand Pipette Stand for 6 pipettes, up to four multi-channels, acrylic

P4406 1/pack
EUR 97.25
Description: SureStand Pipette Stand for 6 pipettes up to four multi-channels

SureStand Pipette Stand for 8 pipettes, up to six multi-channels, acrylic

P4408 1/pack
EUR 102.65
Description: SureStand Pipette Stand for 8 pipettes up to six multi-channels

UNIVERSAL STAND FOR FOUR PIPETTORS, RED

AP-USD4-P 1/pk
EUR 69
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

UNIVERSAL STAND FOR SIX PIPETTORS, TRANSPARENT

AP-USD6 1/pk
EUR 85
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Gel Casting Stand for 5x6cm gels

E1101-CS2 1 PC
EUR 112.85

Gel casting stand L

2322111 5unit
EUR 257
Description: 1pc

Gel casting stand S

2322116 5unit
EUR 257
Description: 1pc

Homogenizer stand for Agile? Hand-held homogenizer

AHM1-VS
EUR 529

stand for 4/6/8 w lamps

VLS6 ea
EUR 200

UNIVERSAL LINEAR 4 PLACE STAND

AP-USD4 1/pk
EUR 70
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

Exo-Flow Multifunctional Magnetic Stand

EXOFLOW700A-1 1 Kit
EUR 402

handheld stand for 4/6/8 w lamps

VLSMA ea
EUR 124

PIPET CONTROLLER - 2-POSITION CHARGING STAND

357490 1/pk
EUR 64
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories

Circulating DNA Kit (with Magnetic Stand)

abx098869-50rxns 50 rxns
EUR 425

Circulating DNA Kit (with Magnetic Stand)

abx298026-50rxns 50 rxns
EUR 425

Wire Rack Stand for 8.5 x 11" autoclave bags

A9004-WR 1/pack
EUR 65.53
Description: Stand For Autoclave Bag

Blood Genomic DNA Kit (with Magnetic Stand)

abx098867-50rxns 50 rxns
EUR 300

Viral DNA / RNA Kit (with Magnetic Stand)

abx098871-50rxns 50 rxns
EUR 398

Viral DNA/RNA Kit (with Magnetic Stand)

abx298029-50rxns 50 rxns
EUR 398

MagneSphere(R) Mag. Sep. Stand 12-hole, 12x75mm

Z5343 unit
EUR 359.4

Reversible Combs, 22/12 teeth, for E1101-CS1 Casting Stand, pk 2

BM0287 Ea
EUR 58

Reversible Combs, 18/10 teeth, for E1101-CS2 Casting Stand, pk 2

BM0288 Ea
EUR 58

Shelf

H1010-SH 1 PC
EUR 1413.5

SHELF, FULL FOR 6750 &6752

480157 1/pk
EUR 132
Description: Lab Equipment; Constant Temperature Equipment

SHELF, FULL FOR 6753&6754

480170 1/pk
EUR 132
Description: Lab Equipment; Constant Temperature Equipment

Extra Shelf

H2265-SH 1 PC
EUR 1339.55

EXTRA SHELF

H2300-SH 1 PC
EUR 323.39

Extra Shelf

H2505-130SH 1 PC
EUR 386.03

Extra Shelf

H2505-40SH 1 PC
EUR 155.48

Extra Shelf

H2505-70SH 1 PC
EUR 155.48

Gel Casting Stand for 5x6cm gels, includes 4 trays, and 2 combs (18/10 teeth)

BM0290 Ea
EUR 116

TIPS,200UL,NAT GRAD STAND VACUUM FORMED UNITS 10 DECKS,960/3840

4126 960/pk
EUR 143
Description: Non Filter Tips; DeckWork Non Filter Tips

TIPS,200UL,YEL GRADUAYED STAND VACUUM FORMED UNITS 10 DECKS,960/3840

4127 960/pk
EUR 143
Description: Non Filter Tips; DeckWork Non Filter Tips

Gel Casting set for 10.5x6cm gels, includes stand, 2 trays, and 2 combs(22/12 teeth)

BM0289 Set
EUR 93

PETRI DISH SHELF

480145 1/pk
EUR 132
Description: Lab Equipment; Constant Temperature Equipment

Mounting Media

99-MED 3 ml
EUR 211
Description: Mounting media for use in microscopy preparations

AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS

AP-STR-KIT-P 1/pk
EUR 721
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

VIAL,CRYO,INT THREAD,2ML,ROUND,SILICONE WASHER,SELF-STAND,S,50/250

431386 50/pk
EUR 135
Description: Cryogenic Vials; Corning Cryogenic Vials

TIPS,200UL NAT GRAD LOW BIND STAND IN HINGED RACKS,NS,BK,960/3840

4148 960/pk
EUR 256
Description: Non Filter Tips; DeckWork Non Filter Tips

TIPS,200UL NAT GRAD LOW BIND STAND IN HINGED RACKS,S,BK,960/3840

4151 960/pk
EUR 278
Description: Non Filter Tips; DeckWork Non Filter Tips

Fine natural hair brush for mounting sections

PO501 each
EUR 67

UNIVERSAL LINEAR STAND FOR FOUR PIPETTORS, ORANGE (FOUR SINGLE-CHANNEL, MAXIMUM OF TWO 8-CHANNEL OR 12-CHANNEL PIPETTORS)

6066 1/pk
EUR 69
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors

UNIVERSAL LINEAR STAND FOR FOUR PIPETTORS, TRANSPARENT (FOUR SINGLE-CHANNEL, MAXIMUM OF TWO 8-CHANNEL OR 12-CHANNEL PIPETTORS)

6067 1/pk
EUR 69
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors

UNIVERSAL LINEAR STAND FOR SIX PIPETTORS, TRANSPARENT (SIX SINGLE-CHANNEL, MAXIMUM OF THREE 8-CHANNEL OR 12-CHANNEL PIPETTORS)

6068 1/pk
EUR 85
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors

FALCON® PIPETTE CONTROLLER - STANDARD VERSION WITH TWO 0.2UM FILTERS AND TWO 0.45UM FILTERS, 2-POSITION CHARGING STAND, UNIVERSAL POWER SUPPLY AND SET OF 3 BATTERIES

357469 1/pk
EUR 203
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories

FALCON® PIPETTE CONTROLLER - US VERSION WITH TWO 0.2UM FILTERS AND TWO 0.45UM FILTERS, 2-POSITION CHARGING STAND, UNIVERSAL POWER SUPPLY AND SET OF 3 BATTERIES

357471 1/pk
EUR 203
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories

Aqueous Mounting Medium

AR1018 10mL X5 (Enough for 800-1200 assays)
EUR 80

Antifade Mounting Medium

AR1109 10mL (for 1000 assays)
EUR 65

EverBrite Mounting Medium

23001 10mL
EUR 178
Description: Minimum order quantity: 1 unit of 10mL

FluoreGuard Mounting Medium

FMM030 30 ml
EUR 96

FluoreGuard Mounting Medium

FMM060 60 ml
EUR 116

FluoreGuard Mounting Medium

FMM500 500 ml
EUR 327

FluoreGuard Mounting Medium

FMM999 1000 ml
EUR 530

SuperMOUNT? Mounting Medium

1211-20
EUR 201

FluorLast? Mounting Media

1213-20
EUR 142

ONE RELOAD-200UL NAT STAND TIPS VACCUM FORMED 10 DECKS, 10 EMPTY RACKS,960/960

4131 960/pk
EUR 69
Description: Non Filter Tips; DeckWork Non Filter Tips

ONE RELOAD-200UL YEL STAND TIPS VACCUM FORMED 10 DECKS, 10 EMPTY RACKS,960/960

4132 960/pk
EUR 69
Description: Non Filter Tips; DeckWork Non Filter Tips

Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 60ml

NB300 60 ml
EUR 427

Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 30ml

NB300-30 30 ml
EUR 276

Advantage Mounting Media, for permanent mounting and Coverslipping IHC & special stains from water, 125ml

NB300A 125 ml
EUR 543

SILICONE PIPETTE HOLDER FOR AXYGEN MOTOPET

MOTO-SP-HLDR 1/pk
EUR 47
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs

Replacement Charger for Electronic Pipette Controller

P6080-RC 1/pack
EUR 69.58
Description: Replacement Charger for Electronic Pipette Controller

Pipette Tips

20-abx090687
  • EUR 84.00
  • EUR 93.00
  • 1000 × 10 ul
  • 1000 × 1 ml

Extra Shelf 10.5 x 8"

H2200-SH 1 PC
EUR 3107.39

TIPS,200UL,NAT GRAD STAND LOW BIND VACUUM FORM UNITS OF 10 DECKS,BK,960/3840

4154 960/pk
EUR 193
Description: Non Filter Tips; DeckWork Non Filter Tips

Fluorguard Antifade Mounting Medium

F001 1 ml
EUR 86

Anti-Fade Mounting Medium

abx090692-10ml 10 ml
EUR 203

EverBrite Hardset Mounting Medium

23003 10mL
EUR 195
Description: Minimum order quantity: 1 unit of 10mL

Permanent Mounting Media (Aqueous)

PMT030 30 ml
EUR 109

Permanent Mounting Media (Aqueous)

PMT999 1000 ml
EUR 759

Buffered Glycerol Mounting Media

GMM003 3 ml
EUR 61

Buffered Glycerol Mounting Media

GMM030 30 ml
EUR 72

Buffered Glycerol Mounting Media

GMM500 500 ml
EUR 183

Buffered Glycerol Mounting Media

GMM999 1000 ml
EUR 260

REPLACEMENT SILICONE PIPETTE HOLDER FOR STRIPETTOR PLUS

4096 1/pk
EUR 47
Description: Corning and Axygen Liquid Handling Equipment; Stripettor Plus Spare Parts

Silicone Nosepiece adapter for Electronic Pipette Controller

P6080-NA 1/pack
EUR 67.01
Description: Silicone Nosepiece adapter for Electronic Pipette Controller

Benchtop Disposable Set, includes metal stand, 5 x 3 x9" (LxWxH) and 400 red 8.5 x11" bags

A9004-SK 1/pack
EUR 116.83
Description: Benchtop Disposal Set

SILICONE PIPETTE HOLDER

357474 1/pk
EUR 47
Description: Falcon Liquid Handling Equipment; Falcon Pipet Controllers and Accessories

SILICONE PIPETTE HOLDER

4993 1/pk
EUR 47
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs

10ml Pipette Tips

P7700-T10M 1 PC
EUR 101.25

5ml Pipette Tips

P7700-T5M 1 PC
EUR 81.68

Replacement power supply for nUVaClean UV Pipette Carousel

P5590-PS 1/pack
EUR 76.33
Description: Replacement power supply for nUVaClean UV Pipette Carousel

EverBrite Mounting Medium with DAPI

23002 10mL
EUR 195
Description: Minimum order quantity: 1 unit of 10mL

ReadiUse™ microscope mounting solution

20009 50 mL
EUR 115

FluoreGuard Mounting Medium (Hard Set)

FMH030 30 ml
EUR 96

FluoreGuard Mounting Medium (Hard Set)

FMH060 60 ml
EUR 116

FluoreGuard Mounting Medium (Hard Set)

FMH500 500 ml
EUR 327

FluoreGuard Mounting Medium (Hard Set)

FMH999 1000 ml
EUR 530

Our experimental outcomes have demonstrated a profitable prospect as amperometric detection in PDMS-based chip and confirmed excessive tolerance to disturbance by exterior motion supplied in the usage of digital micropipette and no floor inactivation from sample-interference.