Single-cell cloning (SCC) is a essential step in producing monoclonal cell strains, that are broadly used as in vitro fashions and for producing proteins with excessive reproducibility for analysis and the manufacturing of therapeutic medicine. In monoclonal cell line technology, the event time could be shortened by validating the monoclonality of the cloned cells.
Nevertheless, the validation course of at the moment requires specialised gear that isn’t available generally biology laboratories. Right here, we report a disposable SCC system, through which single cells could be remoted, validated, and expanded to type monoclonal cell colonies utilizing standard micropipettes and microscopes. The monoclonal cells could be selectively transferred from the SCC chip to standard tradition plates, utilizing a tissue puncher. Utilizing the system, we demonstrated that monoclonal colonies of actin-GFP (inexperienced fluorescent protein) plasmid-transfected A549 cells may very well be fashioned within the system inside 9 days and subsequently transferred to wells in plates for additional enlargement.
This method provides a cheap different to using specialised gear for monoclonal cell technology. Positioning single cells on a stable floor is a vital approach for understanding the mobile features and cell-cell interactions in cell tradition assays. We developed a microfluidic chip for depositing single cells in microwells utilizing a easy micropipette operation. Cells have been delivered to microwells by the meniscus movement of liquid interface.
The residue deposits of cells have been redistributed with air injection, and the remoted single cells have been saved in microwells. Totally different microwell sizes and depths have been studied to guage the trapping chance of cells. Medium alternative and cell viability staining with the remoted single cells have been achieved in microwells. The chip will function a instrument for single-cell patterning in an easy-to-use method.
Single-cell biophysical examine reveals deformability and inside ordering relationship in T cells
Deformability and inside ordering are key options associated to cell operate, notably essential for cells that routinely endure giant deformations, like T cells throughout extravasation and migration. Within the measurement of cell deformability, a substantial variability is usually obtained, masking the identification of potential interrelationships between deformability, inside ordering and cell operate.
We report the event of a single-cell methodology that mixes measurements of living-cell deformability, utilizing micropipette aspiration, and three-dimensional confocal evaluation of the nucleus and cytoskeleton. We present that this single-cell method can function a robust instrument to establish applicable parameters that characterize deformability inside a inhabitants of cells, not readably discernable in population-averaged information. By making use of this single-cell methodology to mouse CD4+ T cells, our outcomes exhibit that the relative dimension of the nucleus, higher than different geometrical or cytoskeletal options, successfully determines the general deformability of the cells throughout the inhabitants.
Vascular permeability is a serious operate of the microvasculature that’s regulated by a number of components together with blood strain, blood viscosity, and endothelial barrier operate. Intravital microscopy has been used to straight assess vascular permeability in vivo, which permits for the correct measurement of endothelial barrier operate in a very physiological hemodynamic context.
Right here, we describe the process for measuring endothelial barrier operate in mouse fashions of cerebral cavernous malformations, together with micropipette preparation, anesthesia, tracheotomy, jugular catheterization, cremaster dissection, imaging, and information evaluation. These animals exhibit a rise in microvessel permeability and irregular vessel morphology, which require particular consideration.

Dynamics of Toxoplasma gondii Oocyst Phagocytosis by Macrophages
Oocysts are the environmentally resistant stage of the protozoan parasite Toxoplasma gondii. They’re accountable for foodborne infections in people and animals worldwide. Infectious oocysts comprise sporozoites that should exit the sporocyst and oocyst partitions to provoke replication of the parasite throughout the host tissues. Given their robustness and resistance to chemical degradation, it’s nonetheless unclear how the oocyst and sporocyst partitions launch the sporozoites.
This course of known as excystation is assumed to happen within the small gut because of the mixed motion of digestive brokers, but to be recognized. By utilizing an oocyst-macrophage co-culture platform, we beforehand demonstrated in vitro that the excystation of sporozoites and their differentiation into replicative tachyzoites may happen in absence of digestive components, following phagocytosis by macrophages. Right here, we additional characterize the dynamics of the oocyst phagocytosis on the single-cell degree by utilizing optical tweezers and micropipette aspiration methods.
Our outcomes present that the oocyst internalization kinetics can fluctuate amongst a given inhabitants of macrophages, however comparable processes and dynamics may very well be noticed. A lot of the cells manipulate oocysts for ~15 min earlier than internalizing them in sometimes 30 min. This course of primarily includes the actin cytoskeleton of the macrophages.
SLS Lab Basics Variable Volume Pipette 20-200uL |
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SLS4912 | Scientific Laboratory Supplies | EACH | EUR 144.78 |
SLS Lab Basics Variable Volume Pipette 500-5000uL |
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SLS4914 | Scientific Laboratory Supplies | EACH | EUR 173.28 |
SLS Lab Basics Variable Volume Pipette 1000-10000uL |
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SLS4916 | Scientific Laboratory Supplies | EACH | EUR 173.28 |
Positive displacement micropipette Acura capillar 846 volume 1 - 2 - 4 - 5 uL |
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DD61945 | Scientific Laboratory Supplies | EACH | EUR 373.92 |
Positive displacement micropipette Acura capillar 846 volume 10 - 20 - 30 - 40 - 50 uL |
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DD61947 | Scientific Laboratory Supplies | EACH | EUR 373.92 |
Positive displacement micropipette Acura capillar 846 volume 60 - 75 - 80 - 90 - 100 uL |
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DD61948 | Scientific Laboratory Supplies | EACH | EUR 373.92 |
Jade Smart? Mix |
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M1113-500 | Biovision | each | EUR 529.2 |
Smart Personal Micro Centrifuge |
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abx725002-1Unit | Abbexa | 1 Unit | EUR 622.8 |
Jade Smart? Mix-iCycler |
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M1114-500 | Biovision | each | EUR 529.2 |
Jade Smart? Mix-ROX |
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M1116-500 | Biovision | each | EUR 529.2 |
Pe Filter For Micropipette Protection |
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DD061968 | Scientific Laboratory Supplies | PK100 | EUR 101.46 |
Jade Smart? Mix-Low ROX |
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M1115-500 | Biovision | each | EUR 529.2 |
96 Fully Skirted Purple Frame |
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PCR1220 | Scientific Laboratory Supplies | PK50 | EUR 165.3 |
96 Fully Skirted Blue Frame |
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PCR1222 | Scientific Laboratory Supplies | PK50 | EUR 163.02 |
96 Fully Skirted Green Frame |
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PCR1224 | Scientific Laboratory Supplies | PK50 | EUR 163.02 |
96 Fully Skirted Red Frame |
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PCR1226 | Scientific Laboratory Supplies | PK50 | EUR 163.02 |
96 Fully Skirted Black Frame |
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PCR1228 | Scientific Laboratory Supplies | PK50 | EUR 163.02 |
96 Fully Skirted White Frame |
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PCR1230 | Scientific Laboratory Supplies | PK50 | EUR 163.02 |
96 Fully Skirted Clear Frame |
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PCR1232 | Scientific Laboratory Supplies | PK50 | EUR 249.66 |
96 Fully Skirted Rigid Skirt |
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PCR1234 | Scientific Laboratory Supplies | PK50 | EUR 261.06 |
96 Fully Skirted Clear Frame |
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PCR1240 | Scientific Laboratory Supplies | PK20 | EUR 141.36 |
96 Fully Skirted Black Frame |
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PCR1244 | Scientific Laboratory Supplies | PK50 | EUR 280.44 |
96 Fully Skirted Black Frame |
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PCR1246 | Scientific Laboratory Supplies | PK50 | EUR 167.58 |
Autoclavable Pans 324X260X70 Pp |
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6902-3000 | Scientific Laboratory Supplies | EACH | EUR 72.96 |
Nalgene Autoclavable Polypropylene Pans |
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69022000 | Scientific Laboratory Supplies | PK6 | EUR 318.06 |
Nunc V96 PolySorp Autoclavable |
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442587 | Scientific Laboratory Supplies | PK60 | EUR 194.94 |
Cap 16mm Non Autoclavable |
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TIS1552 | Scientific Laboratory Supplies | PK1000 | EUR 357.96 |
Forcep PMP 115mm Autoclavable |
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TWY260 | Scientific Laboratory Supplies | EACH | EUR 3.86 |
Nucleic Acid Instrument |
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Smart-32 | Daan Gene | 1 unit/set | Ask for price |
Micropipette Acura 825 0.1-2 uL Socorex |
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DD61104 | Scientific Laboratory Supplies | EACH | EUR 340.86 |
Micropipette Acura 825 2-20 uL Socorex |
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DD61107 | Scientific Laboratory Supplies | EACH | EUR 313.5 |
Micropipette Acura 825 20-200 uL Socorex |
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DD61110 | Scientific Laboratory Supplies | EACH | EUR 313.5 |
Micropipette Acura 825 100-1000 uL Socorex |
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DD61111 | Scientific Laboratory Supplies | EACH | EUR 313.5 |
Micropipette Acura 835 1-10 uL Socorex |
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DD61113 | Scientific Laboratory Supplies | EACH | EUR 339.72 |
Arium Smart Station UP Water - Benchtop |
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WAT4340 | Scientific Laboratory Supplies | EACH | EUR 2143.2 |
Arium Smart Station Pure Water - Benchtop |
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WAT4344 | Scientific Laboratory Supplies | EACH | EUR 1824 |
Fully CpG Methylated Human Genomic DNA |
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X10000 | EpiGentek | 10 µg/100 µl | Ask for price |
Vortex mixer Mini variable |
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MIX2080 | Scientific Laboratory Supplies | EACH | EUR 184.68 |
Lung Volume Kit |
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6050 | Scientific Laboratory Supplies | EA | EUR 120.84 |
Smart 2X PCR pre-mix Pfu (30ul) |
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9K-002-0038 | Bio Basic | 30uL, 30uL | EUR 697.37 |
SMART Standard tube dispensing cassette 5-pack |
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DIS0118 | Scientific Laboratory Supplies | EACH | EUR 3749.46 |
Arium Smart Station UP Water - Wall Mounted |
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WAT4342 | Scientific Laboratory Supplies | EACH | EUR 2143.2 |
Arium Smart Station Pure Water - Wall Mounted |
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WAT4346 | Scientific Laboratory Supplies | EACH | EUR 1824 |
Synbiosis AutoCOL Fully Automated Colony Counting System |
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COU2020 | Scientific Laboratory Supplies | EACH | EUR 119700 |
Stuart Rotator Variable Speed SB3 |
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MIX1960 | Scientific Laboratory Supplies | EACH | EUR 566.58 |
Vortex Mixer SA8 Variable Speed |
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MIX2082 | Scientific Laboratory Supplies | EACH | EUR 320.34 |
Velocity/Volume Thermo-Anemometer |
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ANE1004 | Scientific Laboratory Supplies | EACH | EUR 142.5 |
Pipetman F20 Fixed Volume |
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F123604 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F200 Fixed Volume |
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F123605 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F1000 Fixed Volume |
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F123606 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F5000 Fixed Volume |
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F123607 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F2 Fixed Volume |
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F123770 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F5 Fixed Volume |
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F123771 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F10 Fixed Volume |
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F123772 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F25 Fixed Volume |
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F123775 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F50 Fixed Volume |
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F123778 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F100 Fixed Volume |
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F123784 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F250 Fixed Volume |
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F123787 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F300 Fixed Volume |
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F123788 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F400 Fixed Volume |
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F123789 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman F500 Fixed Volume |
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F123790 | Scientific Laboratory Supplies | EACH | EUR 174.42 |
Pipetman Large Volume Kit |
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F167400 | Scientific Laboratory Supplies | 1KIT | EUR 490.2 |
Pipetman Micro Volume Kit |
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F167500 | Scientific Laboratory Supplies | EACH | EUR 734.16 |
Workstation for 40 Cryovials blue autoclavable |
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ARG1192 | Scientific Laboratory Supplies | EACH | EUR 56.32 |
Workstation for 40 Cryovials red autoclavable |
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ARG1194 | Scientific Laboratory Supplies | EACH | EUR 56.32 |
SMART 2 small tube plastic tip dispensing cassette |
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DIS0122 | Scientific Laboratory Supplies | EACH | EUR 3756.3 |
SMART 2 small tube metal tip dispensing cassette |
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DIS0126 | Scientific Laboratory Supplies | EACH | EUR 3756.3 |
SMART 2 standard tube dispensing cassette 5-pack |
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DIS0130 | Scientific Laboratory Supplies | EACH | EUR 3756.3 |
Polypropylene variable spacer with multiple notches |
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DD49551 | Scientific Laboratory Supplies | EACH | EUR 1.03 |
Pipettor Variable 1ml for Simplette Straws |
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PIP1500 | Scientific Laboratory Supplies | EACH | EUR 210.9 |
Mat Caps for Square Well Plates, Autoclavable |
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GP803 | GenDepot | 50 mats | EUR 400.8 |
Mat Caps for Round Well Plates, Autoclavable |
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GP804 | GenDepot | 50 mats | EUR 400.8 |
Bijou HW Glass 7ml + almn cap Autoclavable |
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BOT5001 | Scientific Laboratory Supplies | PK288 | EUR 225.44 |
Vane Velocity Volume Thermo-Anemometer |
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ANE1006 | Scientific Laboratory Supplies | EACH | EUR 79.8 |
Gradient Mixer total volume 100ml |
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CSLGM100 | Scientific Laboratory Supplies | EACH | EUR 695.4 |
Gradient Mixer total volume 15ml |
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CSLGM15 | Scientific Laboratory Supplies | EACH | EUR 565.44 |
Gradient Mixer total volume 25ml |
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CSLGM25 | Scientific Laboratory Supplies | EACH | EUR 597.36 |
Gradient Mixer total volume 50ml |
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CSLGM50 | Scientific Laboratory Supplies | EACH | EUR 632.7 |
Gradient Mixer total volume 500ml |
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CSLGM500 | Scientific Laboratory Supplies | EACH | EUR 755.82 |
Dispensette S Fixed-volume 1mL |
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DIS1712 | Scientific Laboratory Supplies | EACH | EUR 408.92 |
Dispensette S Fixed-volume 2mL |
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DIS1714 | Scientific Laboratory Supplies | EACH | EUR 408.92 |
Dispensette S Fixed-volume 10mL |
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DIS1718 | Scientific Laboratory Supplies | EACH | EUR 408.92 |
BTD Volume Rocker Switch 100mL |
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DIS6860 | Scientific Laboratory Supplies | EACH | EUR 18.54 |
EzDrop 1000 Micro-Volume Spectrophotometer |
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SPE1060 | Scientific Laboratory Supplies | EACH | EUR 7497.78 |
Finnpipette F3 Fixed Volume 10ul |
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PIP0110 | Scientific Laboratory Supplies | EACH | EUR 226.86 |
Finnpipette F3 Fixed Volume 25ul |
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PIP0111 | Scientific Laboratory Supplies | EACH | EUR 226.86 |
Finnpipette F3 Fixed Volume 50ul |
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PIP0112 | Scientific Laboratory Supplies | EACH | EUR 226.86 |
Finnpipette F3 Fixed Volume 100ul |
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PIP0113 | Scientific Laboratory Supplies | EACH | EUR 226.86 |
Finnpipette F3 Fixed Volume 250ul |
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PIP0114 | Scientific Laboratory Supplies | EACH | EUR 226.86 |
Finnpipette F3 Fixed Volume 500ul |
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PIP0115 | Scientific Laboratory Supplies | EACH | EUR 226.86 |
Finnpipette F3 Fixed Volume 1000ul |
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PIP0116 | Scientific Laboratory Supplies | EACH | EUR 226.86 |
F2 Fixed Volume Pipette 10ul |
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PIP0272 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 25ul |
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PIP0273 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 50ul |
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PIP0274 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 100ul |
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PIP0275 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 250ul |
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PIP0276 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 500ul |
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PIP0277 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 1000ul |
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PIP0278 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 2ml |
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PIP0279 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 3ml |
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PIP0280 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 5ml |
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PIP0281 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 10ml |
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PIP0282 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 20ul |
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PIP0283 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
F2 Fixed Volume Pipette 200ul |
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PIP0284 | Scientific Laboratory Supplies | EACH | EUR 246.24 |
Pipetman L Micro-Volume Kit |
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F167550 | Scientific Laboratory Supplies | EACH | EUR 826.5 |
Pipetman G Large-volume Kit |
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F167920 | Scientific Laboratory Supplies | EACH | EUR 495.9 |
SMART Small tube plastic tip dispensing cassette 5-pack |
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DIS0108 | Scientific Laboratory Supplies | EACH | EUR 3749.46 |
SMART Small tube metal tip dispensing cassette 5-pack |
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DIS0112 | Scientific Laboratory Supplies | EACH | EUR 3749.46 |
96 Well White PCR Plates, Low Profile, Fully Skirted |
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GP821 | GenDepot | 10 plates | EUR 174 |
Portwest A300 Blue Nitrile fully Coated Knitwrist Glove - Medium |
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SAF1542 | Scientific Laboratory Supplies | PK12 | EUR 32.49 |
Portwest A300 Blue Nitrile fully Coated Knitwrist Glove - Large |
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SAF1544 | Scientific Laboratory Supplies | PK12 | EUR 32.49 |
Portwest A300 Blue Nitrile fully Coated Knitwrist Glove - XXL |
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SAF1548 | Scientific Laboratory Supplies | PK12 | EUR 32.49 |
EchoTherm IN35 Chilling/Heating Incubator; with fully programmable control |
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TPS-IN35 | MiTeGen | 1 INCUBATOR | EUR 3950 |
Description: EchoTherm IN35 Chilling/Heating Incubator; with fully programmable control |
EchoTherm IN45 Chilling/Heating Incubator; with fully programmable control |
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TPS-IN45 | MiTeGen | 1 INCUBATOR | EUR 4910 |
Description: EchoTherm IN45 Chilling/Heating Incubator; with fully programmable control |
EchoTherm IN55 Chilling/Heating Incubator; with fully programmable control |
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TPS-IN55 | MiTeGen | 1 INCUBATOR | EUR 7840 |
Description: EchoTherm IN55 Chilling/Heating Incubator; with fully programmable control |
BIOSIGMA transfer pipette - rod volume 1 mL (bulb volume 4.5 mL) graduated bulk |
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DD390509 | Scientific Laboratory Supplies | PK500 | EUR 15.96 |
Capillary micropipette Microcaps Drummond 0.5ulger) for pipettes 1 to 100 mL |
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DD075071 | Scientific Laboratory Supplies | PK100 | EUR 29.64 |
Recombinant human Immunoglobulin heavy variable 3-23 |
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P1883 | FN Test | 100ug | Ask for price |
Description: Recombinant protein for human Immunoglobulin heavy variable 3-23 |
Eppendorf Xplorer single- channel variable 1 -20uL |
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E4861000017 | Scientific Laboratory Supplies | EACH | EUR 629.28 |
Eppendorf Xplorer single- channel variable 10 - 200uL |
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E4861000027 | Scientific Laboratory Supplies | EACH | EUR 629.28 |
Eppendorf Xplorer single- channel variable 0.125 - 2.5mL |
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E4861000044 | Scientific Laboratory Supplies | EACH | EUR 629.28 |
Accuris NextPette Variable Vol Pipette 0.1-1.0uL |
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PIP1340 | Scientific Laboratory Supplies | EACH | EUR 344.28 |
Accuris NextPette Variable Vol Pipette 0.5-10uL |
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PIP1341 | Scientific Laboratory Supplies | EACH | EUR 312.36 |
Accuris NextPette Variable Vol Pipette 2-20uL |
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PIP1342 | Scientific Laboratory Supplies | EACH | EUR 312.36 |
Accuris NextPette Variable Vol Pipette 10-100uL |
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PIP1343 | Scientific Laboratory Supplies | EACH | EUR 312.36 |
Accuris NextPette Variable Vol Pipette 20-200uL |
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PIP1344 | Scientific Laboratory Supplies | EACH | EUR 312.36 |
Accuris NextPette Variable Vol Pipette 100-1000uL |
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PIP1345 | Scientific Laboratory Supplies | EACH | EUR 312.36 |
Accuris NextPette Variable Vol Pipette 1-5mL |
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PIP1346 | Scientific Laboratory Supplies | EACH | EUR 344.28 |
Accuris NextPette Variable Vol Pipette 1-10mL |
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PIP1347 | Scientific Laboratory Supplies | EACH | EUR 344.28 |
Nalgene Safety Wash Bottles 1L Narrow Mouth Autoclavable |
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24051000 | Scientific Laboratory Supplies | PK12 | EUR 214.32 |
Portwest A300 Blue Nitrile fully Coated Knitwrist Glove - Extra Large |
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SAF1546 | Scientific Laboratory Supplies | PK12 | EUR 32.49 |
Sterile bottle container - volume 250 mL |
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DD978220 | Scientific Laboratory Supplies | PK24 | EUR 209.76 |
Sterile bottle container - volume 500 mL |
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DD978221 | Scientific Laboratory Supplies | PK24 | EUR 224.58 |
BTD Volume Rocker Switch 2.5-50mL |
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DIS6859 | Scientific Laboratory Supplies | EACH | EUR 16.21 |
Kinesis Micro-Volume BPR PEEK Tubing |
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CHR3621 | Scientific Laboratory Supplies | EACH | EUR 315.78 |
Kinesis Micro-Volume BPR 500 psi |
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CHR3623 | Scientific Laboratory Supplies | EACH | EUR 315.78 |
New high volume flocell includes probe |
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HOM2652 | Scientific Laboratory Supplies | EACH | EUR 6942.6 |
Low volume flocell probe not included |
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HOM2686 | Scientific Laboratory Supplies | EACH | EUR 1772.7 |
Finnpipette F3 Fixed Volume 1ul Micro |
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PIP0108 | Scientific Laboratory Supplies | EACH | EUR 224.58 |
Finnpipette F3 Fixed Volume 5ul Micro |
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PIP0109 | Scientific Laboratory Supplies | EACH | EUR 224.58 |
Hettich Centrifuge Universal 320 Small Volume |
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CEN0401 | Scientific Laboratory Supplies | EACH | EUR 4450.56 |
Hettich Centrifuge Rotofix 32 Small Volume |
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CEN0408 | Scientific Laboratory Supplies | EACH | EUR 2779.32 |
Hettich Centrifuge Rotanta 460 Large Volume |
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CEN0701 | Scientific Laboratory Supplies | EACH | EUR 8700.48 |
DURAN(R) PRESSURE PLUS BOTTLES VOLUME |
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Z742281-10EA | Scientific Laboratory Supplies | PK10 | EUR 209.76 |
Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 419L |
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FRE2080 | Scientific Laboratory Supplies | EACH | EUR 13221.72 |
Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 579L |
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FRE2082 | Scientific Laboratory Supplies | EACH | EUR 16910.76 |
Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 729L |
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FRE2084 | Scientific Laboratory Supplies | EACH | EUR 18910.32 |
Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 829L |
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FRE2086 | Scientific Laboratory Supplies | EACH | EUR 19686.66 |
LAMBDA PLUS SINGLE CHANNEL PIPETTOR,0.2 TO 2UL,AUTOCLAVABLE |
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4070 | CORNING | 1/pk | EUR 218.4 |
Description: Corning and Axygen Liquid Handling Equipment; Corning Lambda Plus Single Channel Pipettors |
Liberated sporozoites inside macrophages then differentiate into tachyzoites inside 4-6 h following oocyst-macrophage contact. Tachyzoites seem to develop higher in macrophages challenged with free sporocysts or sporozoites than with complete oocysts, suggesting that opening of the oocyst wall is likely one of the most limiting steps for sporozoite excystation completion.