Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level

Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level

Single cell MS (SCMS) methods are underneath speedy growth for molecular evaluation of particular person cells amongst heterogenous populations. Lipids are primary mobile constituents enjoying important capabilities in power storage and mobile signaling processes of cells. Unsaturated lipids are characterised with one or a number of carbon-carbon double (C=C) bonds, and they’re important for cell capabilities and human illnesses.

Characterizing unsaturated lipids in single cells permits for higher understanding of metabolomic biomarkers and therapeutic targets of uncommon cells (e.g., most cancers stem cells); nonetheless, these research stay difficult. We developed a brand new approach utilizing micropipette needle, during which Paternò-Büchi (PB) reactions at C=C bond will be induced, to find out lo-cations of C=C bonds in unsaturated lipids on the single-cell degree. The micropipette needle is produced by combining a pulled glass capillary needle with a fused silica capillary.

Cell lysis solvent and PB reagent (acetone or benzophenone) are delivered into the micropipette needle (tip dimension ~15 um) by means of a fused silica capillary. The capillary needle performs a number of capabilities (i.e., single cell sampling probe, cell lysis container, micro-reactor, and nano-ESI emitter) within the experiments. Each common (no response) and reactive (with PB response) SCMS analyses of the identical cell will be achieved. C=C bond areas had been decided from MS scan and MS/MS of PB merchandise assisted by Python packages.

This system will be probably used for different reactive SCMS research to reinforce molecular evaluation for broad ranges of single cells. One of many attribute options of many marine dinoflagellates is their bioluminescence, which lights up nighttime breaking waves or seawater sliced by a ship’s prow. Whereas the inner biochemistry of sunshine manufacturing by these microorganisms is properly established, the style by which fluid shear or mechanical forces set off bioluminescence continues to be poorly understood.

We report managed measurements of the relation between mechanical stress and light-weight manufacturing on the single cell degree, utilizing high-speed imaging of micropipette-held cells of the marine dinoflagellate Pyrocystis lunula subjected to localized fluid flows or direct indentation. We discover a viscoelastic response during which gentle depth depends upon each the amplitude and charge of deformation, per the motion of stretch-activated ion channels. A phenomenological mannequin captures the experimental observations.

Ethanol antagonizes P2X4 receptors in ventral tegmental space neurons

P2X4 receptors are discovered all through the central nervous system, and research have proven that these purinergic receptors are necessary regulators of alcohol consumption. The ventral tegmental space (VTA) is a crucial area for the rewarding and reinforcing properties of alcohol, however the function of P2X4 receptors on this area is unknown. Utilizing each immunohistochemical and electrophysiological strategies, we examined the interplay between P2X4 receptors and alcohol on VTA neurons.

Incubation of mind slices containing the VTA for two h with siRNA concentrating on P2X4 receptors resulted in a few 25% discount in P2X4 immunoreactivity in tyrosine hydroxylase constructive VTA neurons. In electrophysiological experiments, ATP (0.5-Three mM) produced a discount within the spontaneous firing charge, and ethanol considerably decreased this inhibition. Publicity to siP2X4 for two h by way of the recording micropipette resulted in a suppression of the response of VTA neurons to ATP, however no vital discount within the ethanol inhibition of the ATP response was noticed after this P2X4 downregulation.

These outcomes help the concept VTA neurons are inhibited by ATP, ethanol antagonizes this inhibition, and the ethanol-sensitive part of ATP inhibition is mediated by P2X4 receptors. This interplay of ethanol with P2X4 receptors could also be an necessary regulator of the rewarding results of ethanol, making P2X4 receptors an intriguing goal for the event of brokers to deal with alcohol use problems.

 Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level
Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level

Low-Invasive Cell Injection based mostly on Rotational Microrobot

The development of cell injections has created a necessity for correct, environment friendly, and low-invasive injections. Nonetheless, the standard approaches to cut back cell injury throughout penetration, primarily optimization of micropipette ideas and vision-based computerized injections, have virtually reached the restrict. Right here, described are the design and implementation of a robotic-aided rotatory microinjection system to cut back cell deformation and penetration pressure through the injection.

The homocentric rotation expertise integrates an ultraprecise manipulation system with a number of levels of freedom, which tremendously diminishes the injury to the cell. By means of systematic assessments on zebrafish embryo microinjection, in contrast with conventional easy cell injection methods, the rotary cell injection strategy is ready to scale back the penetration deformation and pressure of the specimen as much as 30%.

Positive displacement micropipette Acura capillar 846 volume 10 - 20 - 30 - 40 - 50 uL

DD61947 EACH
EUR 477.6

Pe Filter For Micropipette Protection

DD061968 PK100
EUR 135.6

Micropipette Acura 825 0.1-2 uL Socorex

DD61104 EACH
EUR 434.4

Micropipette Acura 825 2-20 uL Socorex

DD61107 EACH
EUR 400.8

Micropipette Acura 825 20-200 uL Socorex

DD61110 EACH
EUR 400.8

Micropipette Acura 825 100-1000 uL Socorex

DD61111 EACH
EUR 400.8

Micropipette Acura 835 1-10 uL Socorex

DD61113 EACH
EUR 433.2

Capillary micropipette Microcaps Drummond 0.5ulger) for pipettes 1 to 100 mL

DD075071 PK100
EUR 44.4

Stand for 4 SLS Micropipettes

SLS4938 EACH
EUR 69.2

stand for 6 SLS Micropipettes

SLS4940 EACH
EUR 57.32

Economical tip 100 - 1000 uL for micropipettes

DD391451 PK500
EUR 2010

Economical tip 0.5 - 10 uL for micropipettes

DD391453 PK500
EUR 1485.6

Economical tip 2 - 200 uL for micropipettes

DD391455 PK500
EUR 1485.6

Finnpipette F3 Fixed Volume 1ul Micro

PIP0108 EACH
EUR 254.4

Finnpipette F3 Fixed Volume 5ul Micro

PIP0109 EACH
EUR 254.4

F2 Fixed Volume Pipette 1ul micro tip

PIP0270 EACH
EUR 280.8

F2 Fixed Volume Pipette 5ul micro tip

PIP0271 EACH
EUR 280.8

Pipetman Micro Volume Kit

F167500 EACH
EUR 915.23

Greiner 384 well microplate low volume white HiBase

G784075 PK40
EUR 295.2

Nichrome Wire Microloop 1.5mm volume, 1µl

24660-1 25each
EUR 110

Smart Personal Micro Centrifuge

abx725002-1Unit 1 Unit
EUR 622.8

Corning Chemically Resistant Sealing Mat for 96 Well Expanded Volume Microplate Nonsterile

3346 PK50
EUR 510

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; V-SHAPE; 7MM DIA; 220uL TOTAL VOLUME 100/PK

60180-P333 PK100
EUR 4170

MICROPLATE; PP; 384 POSITIONS; GLASS COATED; HEIGHT 14.4MM; SQUARE-ROUNDED; 145uL TOTAL VOLUME 100/PK

60180-P341 PK100
EUR 5278.8

Microtube with firm closing volume 2 mL amber

DD840614 PK500
EUR 57.6

Corning Low Volume 384 Well White Flat Bottom NBS Microplate 10 per Bag without Lid Nonsterile

3824 PK50
EUR 626.4

Corning Low Volume 384 Well Black Flat Bottom NBS Microplate 10 per Bag without Lid Nonsterile

3820 PK50
EUR 588

Corning Low Volume 384 Well Black Flat Bottom Not Treated Microplate 10 /bag w/out Lid Nonsterile

3821 PK50
EUR 564

Corning Low Volume 384 Well Black Flat Bottom TC-Treated Microplate 10 per Bag with Lid Sterile

3822 PK50
EUR 646.8

Corning Low Volume 384 Well Black with Clear Flat Bottom NBS Microplate 10 /bag w/out Lid Nonsterile

3544 PK50
EUR 777.6

Corning Low Volume 384 Well Black Clear Flat Bottom TC-Treated Microplate 10/bag with Lid Sterile

3542 PK50
EUR 794.4

Corning Low Volume 384 Well Black Clear Flat Bottom UnTreated Microplate 10/bag w/out Lid Nonsterile

3540 PK50
EUR 693.6

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; U-SHAPE; 7MM DIA; 270uL TOTAL VOLUME 10/PK

60180-P330 PK10
EUR 504

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; V-SHAPE; 7MM DIA; 220uL TOTAL VOLUME 10/PK

60180-P332 PK10
EUR 504

MICROPLATE; PP; 384 POSITIONS; GLASS COATED; HEIGHT 14.4MM; SQUARE-ROUNDED; 145uL TOTAL VOLUME 10/PK

60180-P340 PK10
EUR 487.2

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; FLAT BOTTOM; 7MM DIA; 350uL TOTAL VOLUME 10/PK

60180-P334 PK10
EUR 504

Finnpipette F3 Fixed Volume 1000ul

PIP0116 EACH
EUR 255.6

Corning 384 Well Low Volume White Round Bottom NBS Microplate 10 per Bag without Lid Nonsterile

4513 PK50
EUR 837.6

Microtube with firm closing volume 2 mL assorted colours

DD48008 PK500
EUR 57.6

Microtube with firm closing volume 1.5 mL assorted colours

DD840602 PK500
EUR 51.6

Pipetman F2 Fixed Volume

F123770 EACH
EUR 218.04

Pipetman F5 Fixed Volume

F123771 EACH
EUR 218.04

Pipetman F20 Fixed Volume

F123604 EACH
EUR 218.04

Pipetman F10 Fixed Volume

F123772 EACH
EUR 218.04

Pipetman F25 Fixed Volume

F123775 EACH
EUR 218.04

Pipetman F50 Fixed Volume

F123778 EACH
EUR 218.04

Corning 384 Well Microplate Low Volume Black Round Bottom Polystyrene without Lid Non-sterile

4514 PK50
EUR 780

Finnpipette F3 Fixed Volume 100ul

PIP0113 EACH
EUR 255.6

Finnpipette F3 Fixed Volume 250ul

PIP0114 EACH
EUR 255.6

Finnpipette F3 Fixed Volume 500ul

PIP0115 EACH
EUR 255.6

Pipetman F200 Fixed Volume

F123605 EACH
EUR 218.04

Pipetman F100 Fixed Volume

F123784 EACH
EUR 218.04

Pipetman F250 Fixed Volume

F123787 EACH
EUR 218.04

Pipetman F300 Fixed Volume

F123788 EACH
EUR 218.04

Pipetman F400 Fixed Volume

F123789 EACH
EUR 218.04

Pipetman F500 Fixed Volume

F123790 EACH
EUR 218.04

Corning 96 Well Clear High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2481 PK100
EUR 938.4

Corning 96 Well Black High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2483 PK100
EUR 975.6

Corning 96 Well White High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2485 PK100
EUR 1017.6

Pipetman F1000 Fixed Volume

F123606 EACH
EUR 218.04

Pipetman F5000 Fixed Volume

F123607 EACH
EUR 218.04

Corning 96 Well Expanded Volume Not Treated Microplate Standard Height V-Bottom 10 /bag Sterile

3344 PK50
EUR 397.2

Corning 96 Well Black Medium Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2482 PK100
EUR 956.4

Corning 96 Well White Medium Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile

2484 PK100
EUR 997.2

Corning 96 Well Expanded Volume Not Treated Microplate Standard Height V-Bottom 10 /bag Nonsterile

3343 PK50
EUR 348

F2 Fixed Volume Pipette 2ml

PIP0279 EACH
EUR 280.8

F2 Fixed Volume Pipette 3ml

PIP0280 EACH
EUR 280.8

F2 Fixed Volume Pipette 5ml

PIP0281 EACH
EUR 280.8

F2 Fixed Volume Pipette 10ul

PIP0272 EACH
EUR 280.8

F2 Fixed Volume Pipette 25ul

PIP0273 EACH
EUR 280.8

F2 Fixed Volume Pipette 50ul

PIP0274 EACH
EUR 280.8

F2 Fixed Volume Pipette 100ul

PIP0275 EACH
EUR 280.8

F2 Fixed Volume Pipette 250ul

PIP0276 EACH
EUR 280.8

F2 Fixed Volume Pipette 500ul

PIP0277 EACH
EUR 280.8

F2 Fixed Volume Pipette 1000ul

PIP0278 EACH
EUR 280.8

F2 Fixed Volume Pipette 10ml

PIP0282 EACH
EUR 280.8

F2 Fixed Volume Pipette 20ul

PIP0283 EACH
EUR 280.8

F2 Fixed Volume Pipette 200ul

PIP0284 EACH
EUR 280.8

MICROPLATE, 96 WELL, POLYSTYRENE, HALF AREA, LOW VOLUME, BLACK/CLEAR FLAT BOTTOM, TC-TREATED, NO LID, STERILE, BULK

3887A 25/pk
EUR 880.8
Description: Assay; General Assay - 96 A2

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, TC-TREATED, WITH LID, STERILE, BULK

3826 10/pk
EUR 478.8
Description: Assay; 384 Low Volume

Finnpipette F3 Fixed Volume 10ul

PIP0110 EACH
EUR 255.6

Finnpipette F3 Fixed Volume 25ul

PIP0111 EACH
EUR 255.6

Finnpipette F3 Fixed Volume 50ul

PIP0112 EACH
EUR 255.6

MICROPLATE, 96 WELL, POLYSTYRENE, HALF AREA, LOW VOLUME, WHITE/CLEAR FLAT BOTTOM, NON BINDING SURFACE, NO LID, NONSTERILE, BULK

3994 5/pk
EUR 277.2
Description: Assay; General Assay - 96 A2

Lung Volume Kit

6050 EA
EUR 136.8

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, BLACK, FLAT BOTTOM, ROUND WELLS, NOT TREATED, NO LID, NONSTERILE, WITH GENERIC BAR CODE, BULK

3821BC 10/pk
EUR 440.4
Description: Assay; Barcoded - 384 Well

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, TC-TREATED, WITH LID, STERILE, WITH GENERIC BAR CODE, BULK

3826BC 10/pk
EUR 494.4
Description: Assay; Barcoded - 384 Well

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, NON BINDING SURFACE, NO LID, NONSTERILE, WITH GENERIC BAR CODE, BULK

3824BC 10/pk
EUR 646.8
Description: Assay; Barcoded - 384 Well

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, BLACK/CLEAR FLAT BOTTOM, ROUND WELLS, POLY-D-LYSINE, WITH LID, ASEPTICALLY MANUFACTURED, BULK

3643 10/pk
EUR 3554.4
Description: Assay; Coated 384 Well Plt

Hettich Centrifuge Universal 320 Small Volume

CEN0401 EACH
EUR 5070

Hettich Centrifuge Rotofix 32 Small Volume

CEN0408 EACH
EUR 3266.4

Gradient Mixer total volume 100ml

CSLGM100 EACH
EUR 805.2

Gradient Mixer total volume 500ml

CSLGM500 EACH
EUR 876

Pipetman Large Volume Kit

F167400 1KIT
EUR 611.02

Finnpipette F2 Variable Volume. 100-

Z678341-1EA EACH
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BIOSIGMA transfer pipette - rod volume 1 mL (bulb volume 4.5 mL) graduated bulk

DD390509 PK500
EUR 20.4

Jade Smart? Mix

M1113-500 each
EUR 529.2

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, NOT TREATED, FOR HOMOGENOUS LUMINESCENT AND HTRF ASSAYS ONLY, NO LID, NONSTERILE, BULK

3825 10/pk
EUR 418.8
Description: Assay; 384 Low Volume

Sterile bottle container - volume 250 mL

DD978220 PK24
EUR 252

Sterile bottle container - volume 500 mL

DD978221 PK24
EUR 270

BTD Volume Rocker Switch 100mL

DIS6860 EACH
EUR 20.4

Velocity/Volume Thermo-Anemometer

ANE1004 EACH
EUR 158.4

Gradient Mixer total volume 15ml

CSLGM15 EACH
EUR 655.2

Gradient Mixer total volume 25ml

CSLGM25 EACH
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CSLGM50 EACH
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BRAND(TM) SCOOP VOLUME 1000 ML L 400 M

BR139040-6EA PK6
EUR 73.2

BTD Volume Rocker Switch 2.5-50mL

DIS6859 EACH
EUR 18

BIOSIGMA transfer pipette - rod volume 1 mL (bulb volume 4.5 mL) graduated sterile packs of 5

DD390510 PK1000
EUR 82.8

BIOSIGMA transfer pipette - rod volume 3 mL (bulb volume 4 mL) graduated sterile pack of 1

DD390514 PK1000
EUR 111.6

Tip ClipTip Thermo1000 sterile volume 100 - 1000 uL

DD228380 PK768
EUR 116.4

Hettich Centrifuge Universal 320R Refrigerated Small Volume

CEN0406 EACH
EUR 9403.2

Vane Velocity Volume Thermo-Anemometer

ANE1006 EACH
EUR 88.8

Tip-Tub autoclavable tub filling volume 50-60ml

E0030058607 PK10
EUR 30

Low volume flocell probe not included

HOM2686 EACH
EUR 2694.76

DURAN(R) PRESSURE PLUS BOTTLES VOLUME

Z742281-10EA PK10
EUR 243.6

Jade Smart? Mix-ROX

M1116-500 each
EUR 529.2

New high volume flocell includes probe

HOM2652 EACH
EUR 9805.06

BRAND(R) UV CUVETTES MACRO VOLUME 2.5

BR759125-100EA PK100
EUR 56.4

Syringe Injekt 2 pieces volume 2 mL

DD921001 PK100
EUR 12

Jade Smart? Mix-iCycler

M1114-500 each
EUR 529.2

VOLUME ADAPTER APPLICATOR,S,IND,1/1

3507 1/pk
EUR 2226
Description: Genomics; Genomic Accessories

Hettich Centrifuge Rotanta 460 Large Volume

CEN0701 EACH
EUR 9656.4

Cryotube non-sterile useful volume 2 mL

DD42718 PK100
EUR 240

Cryotube non-sterile useful volume 10 mL

DD42719 PK100
EUR 369.6

Pestle & Mortar Unglazed 100mm dia. 175ml volume

PM100 EACH
EUR 15.6

Pestle & Mortar Unglazed 90mm dia. 150ml volume

PM90 EACH
EUR 18

Tip LTS standard version - non-sterile - bulk - volume 5000 uL

DD712032 PK1000
EUR 284.4

Aspiration pipette sterile - volume 1 mL (bulk)

DD978351 PK400
EUR 188.4

Sonicator cell disruptor Q125 max volume 50ml

HOM2352 EACH
EUR 5262.87

Jade Smart? Mix-Low ROX

M1115-500 each
EUR 529.2

Sonicator cell disruptor Q2000F max volume 30l

HOM2358 EACH
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1000 ug/mL DEF in Acetone Volume: 1mL Ampule

S-1130-AC 1ML
EUR 85.2

1000 ug/mL EPN in Acetone Volume: 1mL Ampule

S-1865-AC 1ML
EUR 194.4

SLS Lab Basics Variable Volume Pipette 0.1-2.5uL

SLS4900 EACH
EUR 189.31

NextPette™ variable volume pipette 0.1 to 1.0ul

P7700-1 1 PC
EUR 297.1

SLS Lab Basics Variable Volume Pipette 0.5-10uL

SLS4902 EACH
EUR 189.31

SLS Lab Basics Variable Volume Pipette 5-50uL

SLS4904 EACH
EUR 189.31

SLS Lab Basics Variable Volume Pipette 10-100uL

SLS4906 EACH
EUR 189.31

SLS Lab Basics Variable Volume Pipette 100-1000uL

SLS4908 EACH
EUR 189.31

SLS Lab Basics Variable Volume Pipette 2-20uL

SLS4910 EACH
EUR 189.31

SLS Lab Basics Variable Volume Pipette 20-200uL

SLS4912 EACH
EUR 189.31

SLS Lab Basics Variable Volume Pipette 500-5000uL

SLS4914 EACH
EUR 225.87

SLS Lab Basics Variable Volume Pipette 1000-10000uL

SLS4916 EACH
EUR 225.87

1000 ug/mL Ethion in Acetone Volume: 1mL Ampule

S-1905-AC 1ML
EUR 106.8

1000 ug/mL Folpet in Acetone Volume: 1mL Ampule

S-2057-AC 1ML
EUR 194.4

NextPette™ variable volume pipette 0.5 to 10ul

P7700-10 1 PC
EUR 270.5

NextPette™ variable volume pipette 10 to 100ul

P7700-100 1 PC
EUR 270.5

NextPette™ variable volume pipette 100 to 1000ul

P7700-1000 1 PC
EUR 270.5

NextPette™ variable volume pipette 2 to 20ul

P7700-20 1 PC
EUR 270.5

NextPette™ variable volume pipette 20 to 200ul

P7700-200 1 PC
EUR 270.5

This work breaks the efficiency restrict of present microinjection methods, and paves a brand new manner for the event of low-invasive cell injection methods. It’s envisioned that this rotary microrobot system can excavate huge functions within the area of biomedicine, equivalent to synthetic fertilization and gene remedy.

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