Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level

Single cell MS (SCMS) methods are underneath speedy growth for molecular evaluation of particular person cells amongst heterogenous populations. Lipids are primary mobile constituents enjoying important capabilities in power storage and mobile signaling processes of cells. Unsaturated lipids are characterised with one or a number of carbon-carbon double (C=C) bonds, and they’re important for cell capabilities and human illnesses.

Characterizing unsaturated lipids in single cells permits for higher understanding of metabolomic biomarkers and therapeutic targets of uncommon cells (e.g., most cancers stem cells); nonetheless, these research stay difficult. We developed a brand new approach utilizing micropipette needle, during which Paternò-Büchi (PB) reactions at C=C bond will be induced, to find out lo-cations of C=C bonds in unsaturated lipids on the single-cell degree. The micropipette needle is produced by combining a pulled glass capillary needle with a fused silica capillary.

Cell lysis solvent and PB reagent (acetone or benzophenone) are delivered into the micropipette needle (tip dimension ~15 um) by means of a fused silica capillary. The capillary needle performs a number of capabilities (i.e., single cell sampling probe, cell lysis container, micro-reactor, and nano-ESI emitter) within the experiments. Each common (no response) and reactive (with PB response) SCMS analyses of the identical cell will be achieved. C=C bond areas had been decided from MS scan and MS/MS of PB merchandise assisted by Python packages.

This system will be probably used for different reactive SCMS research to reinforce molecular evaluation for broad ranges of single cells. One of many attribute options of many marine dinoflagellates is their bioluminescence, which lights up nighttime breaking waves or seawater sliced by a ship’s prow. Whereas the inner biochemistry of sunshine manufacturing by these microorganisms is properly established, the style by which fluid shear or mechanical forces set off bioluminescence continues to be poorly understood.

We report managed measurements of the relation between mechanical stress and light-weight manufacturing on the single cell degree, utilizing high-speed imaging of micropipette-held cells of the marine dinoflagellate Pyrocystis lunula subjected to localized fluid flows or direct indentation. We discover a viscoelastic response during which gentle depth depends upon each the amplitude and charge of deformation, per the motion of stretch-activated ion channels. A phenomenological mannequin captures the experimental observations.

P2X4 receptors are discovered all through the central nervous system, and research have proven that these purinergic receptors are necessary regulators of alcohol consumption. The ventral tegmental space (VTA) is a crucial area for the rewarding and reinforcing properties of alcohol, however the function of P2X4 receptors on this area is unknown. Utilizing each immunohistochemical and electrophysiological strategies, we examined the interplay between P2X4 receptors and alcohol on VTA neurons.

Incubation of mind slices containing the VTA for two h with siRNA concentrating on P2X4 receptors resulted in a few 25% discount in P2X4 immunoreactivity in tyrosine hydroxylase constructive VTA neurons. In electrophysiological experiments, ATP (0.5-Three mM) produced a discount within the spontaneous firing charge, and ethanol considerably decreased this inhibition. Publicity to siP2X4 for two h by way of the recording micropipette resulted in a suppression of the response of VTA neurons to ATP, however no vital discount within the ethanol inhibition of the ATP response was noticed after this P2X4 downregulation.

These outcomes help the concept VTA neurons are inhibited by ATP, ethanol antagonizes this inhibition, and the ethanol-sensitive part of ATP inhibition is mediated by P2X4 receptors. This interplay of ethanol with P2X4 receptors could also be an necessary regulator of the rewarding results of ethanol, making P2X4 receptors an intriguing goal for the event of brokers to deal with alcohol use problems.

The development of cell injections has created a necessity for correct, environment friendly, and low-invasive injections. Nonetheless, the standard approaches to cut back cell injury throughout penetration, primarily optimization of micropipette ideas and vision-based computerized injections, have virtually reached the restrict. Right here, described are the design and implementation of a robotic-aided rotatory microinjection system to cut back cell deformation and penetration pressure through the injection.

The homocentric rotation expertise integrates an ultraprecise manipulation system with a number of levels of freedom, which tremendously diminishes the injury to the cell. By means of systematic assessments on zebrafish embryo microinjection, in contrast with conventional easy cell injection methods, the rotary cell injection strategy is ready to scale back the penetration deformation and pressure of the specimen as much as 30%.

Positive displacement micropipette Acura capillar 846 volume 10 - 20 - 30 - 40 - 50 uL
DD61947	Scientific Laboratory Supplies	EACH	EUR 477.6

Pe Filter For Micropipette Protection
DD061968	Scientific Laboratory Supplies	PK100	EUR 135.6

Micropipette Acura 825 0.1-2 uL Socorex
DD61104	Scientific Laboratory Supplies	EACH	EUR 434.4

Micropipette Acura 825 2-20 uL Socorex
DD61107	Scientific Laboratory Supplies	EACH	EUR 400.8

Micropipette Acura 825 20-200 uL Socorex
DD61110	Scientific Laboratory Supplies	EACH	EUR 400.8

Micropipette Acura 825 100-1000 uL Socorex
DD61111	Scientific Laboratory Supplies	EACH	EUR 400.8

Micropipette Acura 835 1-10 uL Socorex
DD61113	Scientific Laboratory Supplies	EACH	EUR 433.2

Capillary micropipette Microcaps Drummond 0.5ulger) for pipettes 1 to 100 mL
DD075071	Scientific Laboratory Supplies	PK100	EUR 44.4

Stand for 4 SLS Micropipettes
SLS4938	Scientific Laboratory Supplies	EACH	EUR 69.2

stand for 6 SLS Micropipettes
SLS4940	Scientific Laboratory Supplies	EACH	EUR 57.32

Economical tip 100 - 1000 uL for micropipettes
DD391451	Scientific Laboratory Supplies	PK500	EUR 2010

Economical tip 0.5 - 10 uL for micropipettes
DD391453	Scientific Laboratory Supplies	PK500	EUR 1485.6

Economical tip 2 - 200 uL for micropipettes
DD391455	Scientific Laboratory Supplies	PK500	EUR 1485.6

Finnpipette F3 Fixed Volume 1ul Micro
PIP0108	Scientific Laboratory Supplies	EACH	EUR 254.4

Finnpipette F3 Fixed Volume 5ul Micro
PIP0109	Scientific Laboratory Supplies	EACH	EUR 254.4

F2 Fixed Volume Pipette 1ul micro tip
PIP0270	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 5ul micro tip
PIP0271	Scientific Laboratory Supplies	EACH	EUR 280.8

Pipetman Micro Volume Kit
F167500	Scientific Laboratory Supplies	EACH	EUR 915.23

Greiner 384 well microplate low volume white HiBase
G784075	Scientific Laboratory Supplies	PK40	EUR 295.2

Nichrome Wire Microloop 1.5mm volume, 1µl
24660-1	Polysciences Europe GmbH	25each	EUR 110

Smart Personal Micro Centrifuge
abx725002-1Unit	Abbexa	1 Unit	EUR 622.8

Corning Chemically Resistant Sealing Mat for 96 Well Expanded Volume Microplate Nonsterile
3346	Scientific Laboratory Supplies	PK50	EUR 510

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; V-SHAPE; 7MM DIA; 220uL TOTAL VOLUME 100/PK
60180-P333	Scientific Laboratory Supplies	PK100	EUR 4170

MICROPLATE; PP; 384 POSITIONS; GLASS COATED; HEIGHT 14.4MM; SQUARE-ROUNDED; 145uL TOTAL VOLUME 100/PK
60180-P341	Scientific Laboratory Supplies	PK100	EUR 5278.8

Microtube with firm closing volume 2 mL amber
DD840614	Scientific Laboratory Supplies	PK500	EUR 57.6

Corning Low Volume 384 Well White Flat Bottom NBS Microplate 10 per Bag without Lid Nonsterile
3824	Scientific Laboratory Supplies	PK50	EUR 626.4

Corning Low Volume 384 Well Black Flat Bottom NBS Microplate 10 per Bag without Lid Nonsterile
3820	Scientific Laboratory Supplies	PK50	EUR 588

Corning Low Volume 384 Well Black Flat Bottom Not Treated Microplate 10 /bag w/out Lid Nonsterile
3821	Scientific Laboratory Supplies	PK50	EUR 564

Corning Low Volume 384 Well Black Flat Bottom TC-Treated Microplate 10 per Bag with Lid Sterile
3822	Scientific Laboratory Supplies	PK50	EUR 646.8

Corning Low Volume 384 Well Black with Clear Flat Bottom NBS Microplate 10 /bag w/out Lid Nonsterile
3544	Scientific Laboratory Supplies	PK50	EUR 777.6

Corning Low Volume 384 Well Black Clear Flat Bottom TC-Treated Microplate 10/bag with Lid Sterile
3542	Scientific Laboratory Supplies	PK50	EUR 794.4

Corning Low Volume 384 Well Black Clear Flat Bottom UnTreated Microplate 10/bag w/out Lid Nonsterile
3540	Scientific Laboratory Supplies	PK50	EUR 693.6

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; U-SHAPE; 7MM DIA; 270uL TOTAL VOLUME 10/PK
60180-P330	Scientific Laboratory Supplies	PK10	EUR 504

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; V-SHAPE; 7MM DIA; 220uL TOTAL VOLUME 10/PK
60180-P332	Scientific Laboratory Supplies	PK10	EUR 504

MICROPLATE; PP; 384 POSITIONS; GLASS COATED; HEIGHT 14.4MM; SQUARE-ROUNDED; 145uL TOTAL VOLUME 10/PK
60180-P340	Scientific Laboratory Supplies	PK10	EUR 487.2

MICROPLATE; PP; 96 POSITIONS; GLASS COATED; HEIGHT 14.6MM; FLAT BOTTOM; 7MM DIA; 350uL TOTAL VOLUME 10/PK
60180-P334	Scientific Laboratory Supplies	PK10	EUR 504

Finnpipette F3 Fixed Volume 1000ul
PIP0116	Scientific Laboratory Supplies	EACH	EUR 255.6

Corning 384 Well Low Volume White Round Bottom NBS Microplate 10 per Bag without Lid Nonsterile
4513	Scientific Laboratory Supplies	PK50	EUR 837.6

Microtube with firm closing volume 2 mL assorted colours
DD48008	Scientific Laboratory Supplies	PK500	EUR 57.6

Microtube with firm closing volume 1.5 mL assorted colours
DD840602	Scientific Laboratory Supplies	PK500	EUR 51.6

Pipetman F2 Fixed Volume
F123770	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F5 Fixed Volume
F123771	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F20 Fixed Volume
F123604	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F10 Fixed Volume
F123772	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F25 Fixed Volume
F123775	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F50 Fixed Volume
F123778	Scientific Laboratory Supplies	EACH	EUR 218.04

Corning 384 Well Microplate Low Volume Black Round Bottom Polystyrene without Lid Non-sterile
4514	Scientific Laboratory Supplies	PK50	EUR 780

Finnpipette F3 Fixed Volume 100ul
PIP0113	Scientific Laboratory Supplies	EACH	EUR 255.6

Finnpipette F3 Fixed Volume 250ul
PIP0114	Scientific Laboratory Supplies	EACH	EUR 255.6

Finnpipette F3 Fixed Volume 500ul
PIP0115	Scientific Laboratory Supplies	EACH	EUR 255.6

Pipetman F200 Fixed Volume
F123605	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F100 Fixed Volume
F123784	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F250 Fixed Volume
F123787	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F300 Fixed Volume
F123788	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F400 Fixed Volume
F123789	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F500 Fixed Volume
F123790	Scientific Laboratory Supplies	EACH	EUR 218.04

Corning 96 Well Clear High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile
2481	Scientific Laboratory Supplies	PK100	EUR 938.4

Corning 96 Well Black High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile
2483	Scientific Laboratory Supplies	PK100	EUR 975.6

Corning 96 Well White High Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile
2485	Scientific Laboratory Supplies	PK100	EUR 1017.6

Pipetman F1000 Fixed Volume
F123606	Scientific Laboratory Supplies	EACH	EUR 218.04

Pipetman F5000 Fixed Volume
F123607	Scientific Laboratory Supplies	EACH	EUR 218.04

Corning 96 Well Expanded Volume Not Treated Microplate Standard Height V-Bottom 10 /bag Sterile
3344	Scientific Laboratory Supplies	PK50	EUR 397.2

Corning 96 Well Black Medium Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile
2482	Scientific Laboratory Supplies	PK100	EUR 956.4

Corning 96 Well White Medium Bind Low Volume Stripwell Microplate 25 per Bag without Lid Nonsterile
2484	Scientific Laboratory Supplies	PK100	EUR 997.2

Corning 96 Well Expanded Volume Not Treated Microplate Standard Height V-Bottom 10 /bag Nonsterile
3343	Scientific Laboratory Supplies	PK50	EUR 348

F2 Fixed Volume Pipette 2ml
PIP0279	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 3ml
PIP0280	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 5ml
PIP0281	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 10ul
PIP0272	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 25ul
PIP0273	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 50ul
PIP0274	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 100ul
PIP0275	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 250ul
PIP0276	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 500ul
PIP0277	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 1000ul
PIP0278	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 10ml
PIP0282	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 20ul
PIP0283	Scientific Laboratory Supplies	EACH	EUR 280.8

F2 Fixed Volume Pipette 200ul
PIP0284	Scientific Laboratory Supplies	EACH	EUR 280.8

MICROPLATE, 96 WELL, POLYSTYRENE, HALF AREA, LOW VOLUME, BLACK/CLEAR FLAT BOTTOM, TC-TREATED, NO LID, STERILE, BULK
3887A	CORNING	25/pk	EUR 880.8
Description: Assay; General Assay - 96 A2

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, TC-TREATED, WITH LID, STERILE, BULK
3826	CORNING	10/pk	EUR 478.8
Description: Assay; 384 Low Volume

Finnpipette F3 Fixed Volume 10ul
PIP0110	Scientific Laboratory Supplies	EACH	EUR 255.6

Finnpipette F3 Fixed Volume 25ul
PIP0111	Scientific Laboratory Supplies	EACH	EUR 255.6

Finnpipette F3 Fixed Volume 50ul
PIP0112	Scientific Laboratory Supplies	EACH	EUR 255.6

MICROPLATE, 96 WELL, POLYSTYRENE, HALF AREA, LOW VOLUME, WHITE/CLEAR FLAT BOTTOM, NON BINDING SURFACE, NO LID, NONSTERILE, BULK
3994	CORNING	5/pk	EUR 277.2
Description: Assay; General Assay - 96 A2

Lung Volume Kit
6050	Scientific Laboratory Supplies	EA	EUR 136.8

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, BLACK, FLAT BOTTOM, ROUND WELLS, NOT TREATED, NO LID, NONSTERILE, WITH GENERIC BAR CODE, BULK
3821BC	CORNING	10/pk	EUR 440.4
Description: Assay; Barcoded - 384 Well

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, TC-TREATED, WITH LID, STERILE, WITH GENERIC BAR CODE, BULK
3826BC	CORNING	10/pk	EUR 494.4
Description: Assay; Barcoded - 384 Well

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, NON BINDING SURFACE, NO LID, NONSTERILE, WITH GENERIC BAR CODE, BULK
3824BC	CORNING	10/pk	EUR 646.8
Description: Assay; Barcoded - 384 Well

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, BLACK/CLEAR FLAT BOTTOM, ROUND WELLS, POLY-D-LYSINE, WITH LID, ASEPTICALLY MANUFACTURED, BULK
3643	CORNING	10/pk	EUR 3554.4
Description: Assay; Coated 384 Well Plt

Hettich Centrifuge Universal 320 Small Volume
CEN0401	Scientific Laboratory Supplies	EACH	EUR 5070

Hettich Centrifuge Rotofix 32 Small Volume
CEN0408	Scientific Laboratory Supplies	EACH	EUR 3266.4

Gradient Mixer total volume 100ml
CSLGM100	Scientific Laboratory Supplies	EACH	EUR 805.2

Gradient Mixer total volume 500ml
CSLGM500	Scientific Laboratory Supplies	EACH	EUR 876

Pipetman Large Volume Kit
F167400	Scientific Laboratory Supplies	1KIT	EUR 611.02

Finnpipette F2 Variable Volume. 100-
Z678341-1EA	Scientific Laboratory Supplies	EACH	EUR 453.6

BIOSIGMA transfer pipette - rod volume 1 mL (bulb volume 4.5 mL) graduated bulk
DD390509	Scientific Laboratory Supplies	PK500	EUR 20.4

Jade Smart? Mix
M1113-500	Biovision	each	EUR 529.2

MICROPLATE, 384 WELL, POLYSTYRENE, LOW VOLUME, WHITE, FLAT BOTTOM, ROUND WELLS, NOT TREATED, FOR HOMOGENOUS LUMINESCENT AND HTRF ASSAYS ONLY, NO LID, NONSTERILE, BULK
3825	CORNING	10/pk	EUR 418.8
Description: Assay; 384 Low Volume

Sterile bottle container - volume 250 mL
DD978220	Scientific Laboratory Supplies	PK24	EUR 252

Sterile bottle container - volume 500 mL
DD978221	Scientific Laboratory Supplies	PK24	EUR 270

BTD Volume Rocker Switch 100mL
DIS6860	Scientific Laboratory Supplies	EACH	EUR 20.4

Velocity/Volume Thermo-Anemometer
ANE1004	Scientific Laboratory Supplies	EACH	EUR 158.4

Gradient Mixer total volume 15ml
CSLGM15	Scientific Laboratory Supplies	EACH	EUR 655.2

Gradient Mixer total volume 25ml
CSLGM25	Scientific Laboratory Supplies	EACH	EUR 692.4

Gradient Mixer total volume 50ml
CSLGM50	Scientific Laboratory Supplies	EACH	EUR 733.2

BRAND(TM) SCOOP VOLUME 1000 ML L 400 M
BR139040-6EA	Scientific Laboratory Supplies	PK6	EUR 73.2

BTD Volume Rocker Switch 2.5-50mL
DIS6859	Scientific Laboratory Supplies	EACH	EUR 18

BIOSIGMA transfer pipette - rod volume 1 mL (bulb volume 4.5 mL) graduated sterile packs of 5
DD390510	Scientific Laboratory Supplies	PK1000	EUR 82.8

BIOSIGMA transfer pipette - rod volume 3 mL (bulb volume 4 mL) graduated sterile pack of 1
DD390514	Scientific Laboratory Supplies	PK1000	EUR 111.6

Tip ClipTip Thermo1000 sterile volume 100 - 1000 uL
DD228380	Scientific Laboratory Supplies	PK768	EUR 116.4

Hettich Centrifuge Universal 320R Refrigerated Small Volume
CEN0406	Scientific Laboratory Supplies	EACH	EUR 9403.2

Vane Velocity Volume Thermo-Anemometer
ANE1006	Scientific Laboratory Supplies	EACH	EUR 88.8

Tip-Tub autoclavable tub filling volume 50-60ml
E0030058607	Scientific Laboratory Supplies	PK10	EUR 30

Low volume flocell probe not included
HOM2686	Scientific Laboratory Supplies	EACH	EUR 2694.76

DURAN(R) PRESSURE PLUS BOTTLES VOLUME
Z742281-10EA	Scientific Laboratory Supplies	PK10	EUR 243.6

Jade Smart? Mix-ROX
M1116-500	Biovision	each	EUR 529.2

New high volume flocell includes probe
HOM2652	Scientific Laboratory Supplies	EACH	EUR 9805.06

BRAND(R) UV CUVETTES MACRO VOLUME 2.5
BR759125-100EA	Scientific Laboratory Supplies	PK100	EUR 56.4

Syringe Injekt 2 pieces volume 2 mL
DD921001	Scientific Laboratory Supplies	PK100	EUR 12

Jade Smart? Mix-iCycler
M1114-500	Biovision	each	EUR 529.2

VOLUME ADAPTER APPLICATOR,S,IND,1/1
3507	CORNING	1/pk	EUR 2226
Description: Genomics; Genomic Accessories

Hettich Centrifuge Rotanta 460 Large Volume
CEN0701	Scientific Laboratory Supplies	EACH	EUR 9656.4

Cryotube non-sterile useful volume 2 mL
DD42718	Scientific Laboratory Supplies	PK100	EUR 240

Cryotube non-sterile useful volume 10 mL
DD42719	Scientific Laboratory Supplies	PK100	EUR 369.6

Pestle & Mortar Unglazed 100mm dia. 175ml volume
PM100	Scientific Laboratory Supplies	EACH	EUR 15.6

Pestle & Mortar Unglazed 90mm dia. 150ml volume
PM90	Scientific Laboratory Supplies	EACH	EUR 18

Tip LTS standard version - non-sterile - bulk - volume 5000 uL
DD712032	Scientific Laboratory Supplies	PK1000	EUR 284.4

Aspiration pipette sterile - volume 1 mL (bulk)
DD978351	Scientific Laboratory Supplies	PK400	EUR 188.4

Sonicator cell disruptor Q125 max volume 50ml
HOM2352	Scientific Laboratory Supplies	EACH	EUR 5262.87

Jade Smart? Mix-Low ROX
M1115-500	Biovision	each	EUR 529.2

Sonicator cell disruptor Q2000F max volume 30l
HOM2358	Scientific Laboratory Supplies	EACH	EUR 29654.09

1000 ug/mL DEF in Acetone Volume: 1mL Ampule
S-1130-AC	Scientific Laboratory Supplies	1ML	EUR 85.2

1000 ug/mL EPN in Acetone Volume: 1mL Ampule
S-1865-AC	Scientific Laboratory Supplies	1ML	EUR 194.4

SLS Lab Basics Variable Volume Pipette 0.1-2.5uL
SLS4900	Scientific Laboratory Supplies	EACH	EUR 189.31

NextPette™ variable volume pipette 0.1 to 1.0ul
P7700-1	Benchmark Scientific	1 PC	EUR 297.1

SLS Lab Basics Variable Volume Pipette 0.5-10uL
SLS4902	Scientific Laboratory Supplies	EACH	EUR 189.31

SLS Lab Basics Variable Volume Pipette 5-50uL
SLS4904	Scientific Laboratory Supplies	EACH	EUR 189.31

SLS Lab Basics Variable Volume Pipette 10-100uL
SLS4906	Scientific Laboratory Supplies	EACH	EUR 189.31

SLS Lab Basics Variable Volume Pipette 100-1000uL
SLS4908	Scientific Laboratory Supplies	EACH	EUR 189.31

SLS Lab Basics Variable Volume Pipette 2-20uL
SLS4910	Scientific Laboratory Supplies	EACH	EUR 189.31

SLS Lab Basics Variable Volume Pipette 20-200uL
SLS4912	Scientific Laboratory Supplies	EACH	EUR 189.31

SLS Lab Basics Variable Volume Pipette 500-5000uL
SLS4914	Scientific Laboratory Supplies	EACH	EUR 225.87

SLS Lab Basics Variable Volume Pipette 1000-10000uL
SLS4916	Scientific Laboratory Supplies	EACH	EUR 225.87

1000 ug/mL Ethion in Acetone Volume: 1mL Ampule
S-1905-AC	Scientific Laboratory Supplies	1ML	EUR 106.8

1000 ug/mL Folpet in Acetone Volume: 1mL Ampule
S-2057-AC	Scientific Laboratory Supplies	1ML	EUR 194.4

NextPette™ variable volume pipette 0.5 to 10ul
P7700-10	Benchmark Scientific	1 PC	EUR 270.5

NextPette™ variable volume pipette 10 to 100ul
P7700-100	Benchmark Scientific	1 PC	EUR 270.5

NextPette™ variable volume pipette 100 to 1000ul
P7700-1000	Benchmark Scientific	1 PC	EUR 270.5

NextPette™ variable volume pipette 2 to 20ul
P7700-20	Benchmark Scientific	1 PC	EUR 270.5

NextPette™ variable volume pipette 20 to 200ul
P7700-200	Benchmark Scientific	1 PC	EUR 270.5

This work breaks the efficiency restrict of present microinjection methods, and paves a brand new manner for the event of low-invasive cell injection methods. It’s envisioned that this rotary microrobot system can excavate huge functions within the area of biomedicine, equivalent to synthetic fertilization and gene remedy.