Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level

Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level

Single cell MS (SCMS) methods are underneath speedy growth for molecular evaluation of particular person cells amongst heterogenous populations. Lipids are primary mobile constituents enjoying important capabilities in power storage and mobile signaling processes of cells. Unsaturated lipids are characterised with one or a number of carbon-carbon double (C=C) bonds, and they’re important for cell capabilities and human illnesses.

Characterizing unsaturated lipids in single cells permits for higher understanding of metabolomic biomarkers and therapeutic targets of uncommon cells (e.g., most cancers stem cells); nonetheless, these research stay difficult. We developed a brand new approach utilizing micropipette needle, during which Paternò-Büchi (PB) reactions at C=C bond will be induced, to find out lo-cations of C=C bonds in unsaturated lipids on the single-cell degree. The micropipette needle is produced by combining a pulled glass capillary needle with a fused silica capillary.

Cell lysis solvent and PB reagent (acetone or benzophenone) are delivered into the micropipette needle (tip dimension ~15 um) by means of a fused silica capillary. The capillary needle performs a number of capabilities (i.e., single cell sampling probe, cell lysis container, micro-reactor, and nano-ESI emitter) within the experiments. Each common (no response) and reactive (with PB response) SCMS analyses of the identical cell will be achieved. C=C bond areas had been decided from MS scan and MS/MS of PB merchandise assisted by Python packages.

This system will be probably used for different reactive SCMS research to reinforce molecular evaluation for broad ranges of single cells. One of many attribute options of many marine dinoflagellates is their bioluminescence, which lights up nighttime breaking waves or seawater sliced by a ship’s prow. Whereas the inner biochemistry of sunshine manufacturing by these microorganisms is properly established, the style by which fluid shear or mechanical forces set off bioluminescence continues to be poorly understood.

We report managed measurements of the relation between mechanical stress and light-weight manufacturing on the single cell degree, utilizing high-speed imaging of micropipette-held cells of the marine dinoflagellate Pyrocystis lunula subjected to localized fluid flows or direct indentation. We discover a viscoelastic response during which gentle depth depends upon each the amplitude and charge of deformation, per the motion of stretch-activated ion channels. A phenomenological mannequin captures the experimental observations.

Ethanol antagonizes P2X4 receptors in ventral tegmental space neurons

P2X4 receptors are discovered all through the central nervous system, and research have proven that these purinergic receptors are necessary regulators of alcohol consumption. The ventral tegmental space (VTA) is a crucial area for the rewarding and reinforcing properties of alcohol, however the function of P2X4 receptors on this area is unknown. Utilizing each immunohistochemical and electrophysiological strategies, we examined the interplay between P2X4 receptors and alcohol on VTA neurons.

Incubation of mind slices containing the VTA for two h with siRNA concentrating on P2X4 receptors resulted in a few 25% discount in P2X4 immunoreactivity in tyrosine hydroxylase constructive VTA neurons. In electrophysiological experiments, ATP (0.5-Three mM) produced a discount within the spontaneous firing charge, and ethanol considerably decreased this inhibition. Publicity to siP2X4 for two h by way of the recording micropipette resulted in a suppression of the response of VTA neurons to ATP, however no vital discount within the ethanol inhibition of the ATP response was noticed after this P2X4 downregulation.

These outcomes help the concept VTA neurons are inhibited by ATP, ethanol antagonizes this inhibition, and the ethanol-sensitive part of ATP inhibition is mediated by P2X4 receptors. This interplay of ethanol with P2X4 receptors could also be an necessary regulator of the rewarding results of ethanol, making P2X4 receptors an intriguing goal for the event of brokers to deal with alcohol use problems.

 Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level
Combining Mass Spectrometry with Paternò-Büchi Reaction to Determine Double-bond Positions in Lipids at the Single-cell Level

Low-Invasive Cell Injection based mostly on Rotational Microrobot

The development of cell injections has created a necessity for correct, environment friendly, and low-invasive injections. Nonetheless, the standard approaches to cut back cell injury throughout penetration, primarily optimization of micropipette ideas and vision-based computerized injections, have virtually reached the restrict. Right here, described are the design and implementation of a robotic-aided rotatory microinjection system to cut back cell deformation and penetration pressure through the injection.

The homocentric rotation expertise integrates an ultraprecise manipulation system with a number of levels of freedom, which tremendously diminishes the injury to the cell. By means of systematic assessments on zebrafish embryo microinjection, in contrast with conventional easy cell injection methods, the rotary cell injection strategy is ready to scale back the penetration deformation and pressure of the specimen as much as 30%.

Positive displacement micropipette Acura capillar 846 volume 10 - 20 - 30 - 40 - 50 uL

DD61947 EACH
EUR 373.92

Positive displacement micropipette Acura capillar 846 volume 60 - 75 - 80 - 90 - 100 uL

DD61948 EACH
EUR 373.92

Jade Smart? Mix

M1113-500
EUR 529.2

Smart Personal Micro Centrifuge

abx725002-1Unit 1 Unit
EUR 622.8

Jade Smart? Mix-iCycler

M1114-500
EUR 529.2

Jade Smart? Mix-ROX

M1116-500
EUR 529.2

CellBrite Fix 555

30088 1KIT
EUR 675.6
Description: Minimum order quantity: 1 unit of 1KIT

CellBrite Fix 640

30089 1KIT
EUR 675.6
Description: Minimum order quantity: 1 unit of 1KIT

CellBriteâ„¢ Fix 488

30090 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

Pe Filter For Micropipette Protection

DD061968 PK100
EUR 101.46

Jade Smart? Mix-Low ROX

M1115-500
EUR 529.2

Mouse FIX ELISA Kit

EMF0233 96Tests
EUR 625.2

Human FIX ELISA Kit

EHF0233 96Tests
EUR 625.2

Rat FIX ELISA Kit

ERF0233 96Tests
EUR 625.2

Rabbit FIX ELISA Kit

ERTF0233 96Tests
EUR 625.2

Porcine FIX ELISA Kit

EPF0233 96Tests
EUR 625.2

MemBriteâ„¢ Fix 405/430

30092 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 488/515

30093 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 543/560

30094 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 568/580

30095 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 594/615

30096 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 640/660

30097 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 660/680

30098 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 680/700

30099 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

Goat FIX ELISA Kit

EGTF0233 96Tests
EUR 625.2

Bovine FIX ELISA Kit

EBF0233 96Tests
EUR 625.2

Canine FIX ELISA Kit

ECF0233 96Tests
EUR 625.2

Anserini FIX ELISA Kit

EAF0024 96Tests
EUR 625.2

Anserini FIX ELISA Kit

EAF0233 96Tests
EUR 625.2

Nucleic Acid Instrument

Smart-32 1 unit/set Ask for price

Arium Smart Station UP Water - Benchtop

WAT4340 EACH
EUR 2143.2

Arium Smart Station Pure Water - Benchtop

WAT4344 EACH
EUR 1824

Micropipette Acura 825 0.1-2 uL Socorex

DD61104 EACH
EUR 340.86

Micropipette Acura 825 2-20 uL Socorex

DD61107 EACH
EUR 313.5

Micropipette Acura 825 20-200 uL Socorex

DD61110 EACH
EUR 313.5

Micropipette Acura 825 100-1000 uL Socorex

DD61111 EACH
EUR 313.5

Micropipette Acura 835 1-10 uL Socorex

DD61113 EACH
EUR 339.72

CellBrite Fix 555, Trial Size

30088-T 1KIT
EUR 234
Description: Minimum order quantity: 1 unit of 1KIT

CellBrite Fix 640, Trial Size

30089-T 1KIT
EUR 234
Description: Minimum order quantity: 1 unit of 1KIT

CellBriteâ„¢ Fix 488, Trial Size

30090-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

Guinea Pig FIX ELISA Kit

EGF0233 96Tests
EUR 625.2

Lung Volume Kit

6050 EA
EUR 120.84

Smart 2X PCR pre-mix Pfu (30ul)

9K-002-0038 30uL, 30uL
EUR 697.37

SMART Standard tube dispensing cassette 5-pack

DIS0118 EACH
EUR 3749.46

Arium Smart Station UP Water - Wall Mounted

WAT4342 EACH
EUR 2143.2

Arium Smart Station Pure Water - Wall Mounted

WAT4346 EACH
EUR 1824

MemBriteâ„¢ Fix 405/430, Trial Size

30092-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 488/515, Trial Size

30093-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 543/560, Trial Size

30094-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 568/580, Trial Size

30095-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 594/615, Trial Size

30096-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 640/660, Trial Size

30097-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 660/680, Trial Size

30098-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix 680/700, Trial Size

30099-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

Pipetman F20 Fixed Volume

F123604 EACH
EUR 174.42

Pipetman F200 Fixed Volume

F123605 EACH
EUR 174.42

Pipetman F1000 Fixed Volume

F123606 EACH
EUR 174.42

Pipetman F5000 Fixed Volume

F123607 EACH
EUR 174.42

Pipetman F2 Fixed Volume

F123770 EACH
EUR 174.42

Pipetman F5 Fixed Volume

F123771 EACH
EUR 174.42

Pipetman F10 Fixed Volume

F123772 EACH
EUR 174.42

Pipetman F25 Fixed Volume

F123775 EACH
EUR 174.42

Pipetman F50 Fixed Volume

F123778 EACH
EUR 174.42

Pipetman F100 Fixed Volume

F123784 EACH
EUR 174.42

Pipetman F250 Fixed Volume

F123787 EACH
EUR 174.42

Pipetman F300 Fixed Volume

F123788 EACH
EUR 174.42

Pipetman F400 Fixed Volume

F123789 EACH
EUR 174.42

Pipetman F500 Fixed Volume

F123790 EACH
EUR 174.42

Pipetman Large Volume Kit

F167400 1KIT
EUR 490.2

Pipetman Micro Volume Kit

F167500 EACH
EUR 734.16

Velocity/Volume Thermo-Anemometer

ANE1004 EACH
EUR 142.5

SMART 2 small tube plastic tip dispensing cassette

DIS0122 EACH
EUR 3756.3

SMART 2 small tube metal tip dispensing cassette

DIS0126 EACH
EUR 3756.3

SMART 2 standard tube dispensing cassette 5-pack

DIS0130 EACH
EUR 3756.3

Mouse FIX(Coagulation Factor IX) ELISA Kit

EM1026 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

Rat FIX(Coagulation Factor IX) ELISA Kit

ER0954 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.094 ng/ml

Mouse Coagulation Factor IX (FIX) CLIA Kit

abx195599-96tests 96 tests
EUR 990

Rat Coagulation Factor IX (FIX) CLIA Kit

abx195600-96tests 96 tests
EUR 990

SMART Small tube plastic tip dispensing cassette 5-pack

DIS0108 EACH
EUR 3749.46

SMART Small tube metal tip dispensing cassette 5-pack

DIS0112 EACH
EUR 3749.46

Finnpipette F3 Fixed Volume 10ul

PIP0110 EACH
EUR 226.86

Finnpipette F3 Fixed Volume 25ul

PIP0111 EACH
EUR 226.86

Finnpipette F3 Fixed Volume 50ul

PIP0112 EACH
EUR 226.86

Finnpipette F3 Fixed Volume 100ul

PIP0113 EACH
EUR 226.86

Finnpipette F3 Fixed Volume 250ul

PIP0114 EACH
EUR 226.86

Finnpipette F3 Fixed Volume 500ul

PIP0115 EACH
EUR 226.86

Finnpipette F3 Fixed Volume 1000ul

PIP0116 EACH
EUR 226.86

F2 Fixed Volume Pipette 10ul

PIP0272 EACH
EUR 246.24

F2 Fixed Volume Pipette 25ul

PIP0273 EACH
EUR 246.24

F2 Fixed Volume Pipette 50ul

PIP0274 EACH
EUR 246.24

F2 Fixed Volume Pipette 100ul

PIP0275 EACH
EUR 246.24

F2 Fixed Volume Pipette 250ul

PIP0276 EACH
EUR 246.24

F2 Fixed Volume Pipette 500ul

PIP0277 EACH
EUR 246.24

F2 Fixed Volume Pipette 1000ul

PIP0278 EACH
EUR 246.24

F2 Fixed Volume Pipette 2ml

PIP0279 EACH
EUR 246.24

F2 Fixed Volume Pipette 3ml

PIP0280 EACH
EUR 246.24

F2 Fixed Volume Pipette 5ml

PIP0281 EACH
EUR 246.24

F2 Fixed Volume Pipette 10ml

PIP0282 EACH
EUR 246.24

F2 Fixed Volume Pipette 20ul

PIP0283 EACH
EUR 246.24

F2 Fixed Volume Pipette 200ul

PIP0284 EACH
EUR 246.24

Pipetman L Micro-Volume Kit

F167550 EACH
EUR 826.5

Pipetman G Large-volume Kit

F167920 EACH
EUR 495.9

Vane Velocity Volume Thermo-Anemometer

ANE1006 EACH
EUR 79.8

Gradient Mixer total volume 100ml

CSLGM100 EACH
EUR 695.4

Gradient Mixer total volume 15ml

CSLGM15 EACH
EUR 565.44

Gradient Mixer total volume 25ml

CSLGM25 EACH
EUR 597.36

Gradient Mixer total volume 50ml

CSLGM50 EACH
EUR 632.7

Gradient Mixer total volume 500ml

CSLGM500 EACH
EUR 755.82

Dispensette S Fixed-volume 1mL

DIS1712 EACH
EUR 408.92

Dispensette S Fixed-volume 2mL

DIS1714 EACH
EUR 408.92

Dispensette S Fixed-volume 10mL

DIS1718 EACH
EUR 408.92

BTD Volume Rocker Switch 100mL

DIS6860 EACH
EUR 18.54

Finnpipette F2 Variable Volume. 100-

Z678341-1EA EACH
EUR 375.06

EzDrop 1000 Micro-Volume Spectrophotometer

SPE1060 EACH
EUR 7497.78

CLIA kit for Mouse FIX (Coagulation Factor IX)

E-CL-M0207 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Mouse FIX (Coagulation Factor IX) in samples from Serum, Plasma, Cell supernatant

CLIA kit for Rat FIX (Coagulation Factor IX)

E-CL-R0156 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Rat FIX (Coagulation Factor IX) in samples from Serum, Plasma, Cell supernatant

BIOSIGMA transfer pipette - rod volume 1 mL (bulb volume 4.5 mL) graduated bulk

DD390509 PK500
EUR 15.96

Capillary micropipette Microcaps Drummond 0.5ulger) for pipettes 1 to 100 mL

DD075071 PK100
EUR 29.64

FIX ELISA Kit| Rat Coagulation Factor IX ELISA Kit

EF017729 96 Tests
EUR 826.8

FIX ELISA Kit| Mouse Coagulation Factor IX ELISA Kit

EF013600 96 Tests
EUR 826.8

Finnpipette F3 Fixed Volume 1ul Micro

PIP0108 EACH
EUR 224.58

Finnpipette F3 Fixed Volume 5ul Micro

PIP0109 EACH
EUR 224.58

New high volume flocell includes probe

HOM2652 EACH
EUR 6942.6

Low volume flocell probe not included

HOM2686 EACH
EUR 1772.7

Sterile bottle container - volume 250 mL

DD978220 PK24
EUR 209.76

Sterile bottle container - volume 500 mL

DD978221 PK24
EUR 224.58

BTD Volume Rocker Switch 2.5-50mL

DIS6859 EACH
EUR 16.21

Kinesis Micro-Volume BPR PEEK Tubing

CHR3621 EACH
EUR 315.78

Kinesis Micro-Volume BPR 500 psi

CHR3623 EACH
EUR 315.78

Hettich Centrifuge Universal 320 Small Volume

CEN0401 EACH
EUR 4450.56

Hettich Centrifuge Rotofix 32 Small Volume

CEN0408 EACH
EUR 2779.32

Hettich Centrifuge Rotanta 460 Large Volume

CEN0701 EACH
EUR 8700.48

DURAN(R) PRESSURE PLUS BOTTLES VOLUME

Z742281-10EA PK10
EUR 209.76

Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 419L

FRE2080 EACH
EUR 13221.72

Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 579L

FRE2082 EACH
EUR 16910.76

Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 729L

FRE2084 EACH
EUR 18910.32

Haier Biomedical Low Energy Smart Touch Screen -86C ULT Freezer 829L

FRE2086 EACH
EUR 19686.66

BIOSIGMA transfer pipette - rod volume 1 mL (bulb volume 4.5 mL) graduated sterile packs of 5

DD390510 PK1000
EUR 68.4

BIOSIGMA transfer pipette - rod volume 3 mL (bulb volume 4 mL) graduated sterile pack of 1

DD390514 PK1000
EUR 92.34

VOLUME ADAPTER APPLICATOR,S,IND,1/1

3507 1/pk
EUR 2226
Description: Genomics; Genomic Accessories

F2 Fixed Volume Pipette 1ul micro tip

PIP0270 EACH
EUR 246.24

F2 Fixed Volume Pipette 5ul micro tip

PIP0271 EACH
EUR 246.24

Pestle & Mortar Unglazed 100mm dia. 175ml volume

PM100 EACH
EUR 13.68

Pestle & Mortar Unglazed 90mm dia. 150ml volume

PM90 EACH
EUR 15.5

Sonicator cell disruptor Q125 max volume 50ml

HOM2352 EACH
EUR 4101.72

Sonicator cell disruptor Q2000F max volume 30l

HOM2358 EACH
EUR 20965.74

Cryotube non-sterile useful volume 2 mL

DD42718 PK100
EUR 205.2

Cryotube non-sterile useful volume 10 mL

DD42719 PK100
EUR 310.08

Syringe Injekt 2 pieces volume 2 mL

DD921001 PK100
EUR 10.26

Aspiration pipette sterile - volume 1 mL (bulk)

DD978351 PK400
EUR 156.18

This work breaks the efficiency restrict of present microinjection methods, and paves a brand new manner for the event of low-invasive cell injection methods. It’s envisioned that this rotary microrobot system can excavate huge functions within the area of biomedicine, equivalent to synthetic fertilization and gene remedy.

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